DOI: 10.1016/j.scitotenv.2020.136940
论文题名: Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics
作者: Jiao S. ; Nie M. ; Song H. ; Xu D. ; You F.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 715 语种: 英语
英文关键词: Liver
; Low temperature
; Metabolomics
; Nibea albiflora
; Starvation
Scopus关键词: Amino acids
; Biochemistry
; Biomolecules
; Biosynthesis
; Cell death
; Climate change
; Fish
; Liver
; Metabolism
; Peptides
; Physiological models
; Physiology
; Temperature
; Unsaturated fatty acids
; Global climate changes
; Glutathione metabolism
; Glycosylphosphatidylinositol anchors
; Low temperatures
; Metabolomics
; Nibea albiflora
; Sphingolipid metabolism
; Starvation
; Metabolites
; ABC transporter
; arginine
; biological marker
; eicosenoic acid
; galactose
; glutamic acid
; glutathione disulfide
; glycerophospholipid
; glycosylphosphatidylinositol anchored protein
; proline
; purine
; sphingolipid
; unclassified drug
; unsaturated fatty acid
; low temperature
; metabolism
; metabolite
; mortality
; perciform
; physiological response
; starvation
; amino acid metabolism
; animal experiment
; animal tissue
; Article
; autophagy (cellular)
; behavior change
; biological functions
; carbohydrate metabolism
; cold stress
; controlled study
; down regulation
; fatty acid synthesis
; fish
; glutathione metabolism
; growth
; liquid chromatography-mass spectrometry
; low temperature
; metabolomics
; Nibea albiflora
; nonhuman
; overwintering
; phospholipid metabolism
; physiological response
; priority journal
; purine metabolism
; regulatory mechanism
; sphingolipid metabolism
; starvation
; survival
; transcriptomics
; upregulation
; China
; Nibea albiflora
英文摘要: As global climate changes, mass mortality in farmed fish associated with the severely cold weather has aroused growing concerns. Yellow drum (Nibea albiflora) is an important maricultured fish in China, whereby its aquaculture suffered from overwinter mortality associated with cold and cold-induced-fasting stresses. Here, by using LC-MS metabolomics combined with transcriptomics, we investigated the physiological responses of yellow drum liver to cold and starvation stresses. The experiment involved four groups: 16 °C fed group (CG1), 16 °C unfed group (CG2), 8 °C fed group (EG1), and 8 °C unfed group (EG2). Under cold stress, a total of 308 and 257 differential metabolites were identified in EG1 vs. CG1 and EG2 vs. CG2, respectively, showing 5 overlapping significant pathways: glutathione metabolism, biosynthesis of unsaturated fatty acids, galactose metabolism, arginine and proline metabolism, and ABC transporters. Intersection analysis identified that glutamate, oxidized glutathione (GSSG), and eicosenoic acid were the common metabolites induced by cold stress. Under starvation stress, a total of 300 and 215 differential metabolites were identified in CG2 vs. CG1 and EG2 vs. EG1, respectively, showing 2 overlapping significant pathways: glutathione metabolism and galactose metabolism. Intersection analysis revealed that glutamate and GSSG were the common metabolites caused by fasting. Under cold and starvation combined stresses, 286 differential metabolites were identified in EG2 vs. CG1, showing 7 influenced pathways: glycerophospholipid metabolism, biosynthesis of unsaturated fatty acids, glutathione metabolism, sphingolipid metabolism, glycosylphosphatidylinositol (GPI)-anchor biosynthesis, autophagy, and purine metabolism. Interestingly, the glutamate and GSSG were induced by both single and combined stresses of cold and starvation treatments. These findings suggest that glutathione metabolism and its related metabolites (glutamate and GSSG) could be potential biomarkers of cold and starvation stresses in yellow drum. Overall, the results of this study provided insights into the physiological regulation in response to cold and starvation stresses in this fish. © 2020 Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158258
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266237, China; University of Chinese Academy of Sciences, Beijing, 10049, China; Marine Fishery Institute of Zhejiang Province, Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhoushan, 316100, China
Recommended Citation:
Jiao S.,Nie M.,Song H.,et al. Physiological responses to cold and starvation stresses in the liver of yellow drum (Nibea albiflora) revealed by LC-MS metabolomics[J]. Science of the Total Environment,2020-01-01,715