DOI: 10.1016/j.jhazmat.2019.121788
论文题名: Nanoplastics: From tissue accumulation to cell translocation into Mytilus galloprovincialis hemocytes. resilience of immune cells exposed to nanoplastics and nanoplastics plus Vibrio splendidus combination
作者: Sendra M. ; Saco A. ; Yeste M.P. ; Romero A. ; Novoa B. ; Figueras A.
刊名: Journal of Hazardous Materials
ISSN: 3043894
出版年: 2020
卷: 388 语种: 英语
英文关键词: Bioaccumulation
; Cell motility
; Endocytosis routes
; Mussel
; V. splendidus
Scopus关键词: Antigen-antibody reactions
; Bioaccumulation
; Biochemistry
; Cell death
; Molecular biology
; Antimicrobial peptide
; Cell motility
; Endocytosis routes
; Functional parameters
; In-vivo experiments
; Mussel
; Mytilus galloprovincialis
; V. splendidus
; Blood
; clathrin
; myticin c
; nanoplastic
; polypeptide antibiotic agent
; polystyrene
; reactive oxygen metabolite
; bioaccumulation
; bivalve
; cell component
; muscle
; peptide
; plastic
; translocation
; animal experiment
; animal tissue
; apoptosis
; bioaccumulation
; blood cell
; caveola
; cell motility
; endocytosis
; exocrine gland
; exposure
; gene expression
; hemolymph
; histology
; immunocompetent cell
; in vitro study
; in vivo study
; internalization
; muscle
; Mytilus galloprovincialis
; nonhuman
; particle size
; phagocytosis
; plastic waste
; scanning electron microscopy
; transmission electron microscopy
; Vibrio splendidus
; Mytilus galloprovincialis
; Vibrio splendidus
英文摘要: Plastic litter is an issue of global concern. In this work Mytilus galloprovincialis was used to study the distribution and effects of polystyrene nanoplastics (PS NPs) of different sizes (50 nm, 100 nm and 1 μm) on immune cells. Internalization and translocation of NPs to hemolymph were carried out by in vivo experiments, while endocytic routes and effects of PS NPs on hemocytes were studied in vitro. The smallest PS NPs tested were detected in the digestive gland and muscle. A fast and size-dependent translocation of PS NPs to the hemolymph was recorded after 3 h of exposure. The internalization rate of 50 nm PS NPs was lower when caveolae and clathrin endocytosis pathways were inhibited. On the other hand, the internalization of larger particles decreased when phagocytosis was inhibited. The hemocytes exposed to NPs had changes in motility, apoptosis, ROS and phagocytic capacity. However, they showed resilience when were infected with bacteria after PS NP exposure being able to recover their phagocytic capacity although the expression of the antimicrobial peptide Myticin C was reduced. Our findings show for the first time the translocation of PS NPs into hemocytes and how their effects trigger the loss of its functional parameters. © 2019 The Authors Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158402
Appears in Collections: 气候变化与战略
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作者单位: Institute of Marine Research (IIM), National Research Council (CSIC), Eduardo Cabello 6, Vigo, 36208, Spain; Department of Material Science, Metallurgical Engineering and Inorganic Chemistry, University of Cádiz, Spain
Recommended Citation:
Sendra M.,Saco A.,Yeste M.P.,et al. Nanoplastics: From tissue accumulation to cell translocation into Mytilus galloprovincialis hemocytes. resilience of immune cells exposed to nanoplastics and nanoplastics plus Vibrio splendidus combination[J]. Journal of Hazardous Materials,2020-01-01,388