DOI: 10.1016/j.scitotenv.2019.136045
论文题名: The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses
作者: Huo D. ; Sun L. ; Storey K.B. ; Zhang L. ; Liu S. ; Sun J. ; Yang H.
刊名: Science of the Total Environment
ISSN: 489697
出版年: 2020
卷: 709 语种: 英语
英文关键词: Anoxia
; Echinoderm
; Global warming
; Heat
; Non-coding RNA
; RNA sequencing
Scopus关键词: Binding sites
; Genes
; Global warming
; Heating
; Marine biology
; Metabolism
; Polymerase chain reaction
; RNA
; Anoxia
; Co-expression networks
; Echinoderm
; Global climate changes
; Hypoxia-inducible factors
; Luciferase reporter assays
; Non-coding RNAs
; Rna sequencing
; Bioelectric potentials
; hypoxia inducible factor 1alpha
; long untranslated RNA
; messenger RNA
; microRNA
; microRNA 2013 3p
; unclassified drug
; anoxia
; climate change
; echinoderm
; environmental stress
; gene expression
; global change
; hypoxia
; RNA
; Apostichopus japonicus
; Article
; binding site
; cell cycle
; climate change
; controlled study
; down regulation
; energy metabolism
; environmental stress
; gene control
; gene expression
; gene targeting
; hypoxia
; luciferase assay
; nonhuman
; priority journal
; real time polymerase chain reaction
; RNA sequence
; sea cucumber
; temperature stress
; upregulation
; Apostichopus japonicus
; Dioscorea trifida
; Echinodermata
; Holothuroidea
英文摘要: The aquatic environment can be greatly impacted by thermal and hypoxic stresses, particularly caused by intensified global warming. Hence, there is an urgency to understand the response mechanisms of marine organisms to adverse environment. Although long non-coding RNAs (lncRNAs) are involved in many biological processes, their roles in stress responses still remain unclear. Here, differentially expressed (DE) lncRNAs and mRNAs were identified as responses to environmental stresses in the economically important sea cucumber, Apostichopus japonicus, and their potential roles were explored. Based on a total of 159, 355 and 495 significantly upregulated genes and 230, 518 and 647 significantly downregulated genes identified in the thermal, hypoxic and combination thermal + hypoxic stress treatments, respectively, we constructed DE-lncRNA-mRNA coexpression networks. Among the networks, eight shared pairs were identified from the three treatments, and based on the connectivity degree, MSTRG.27265, MSTRG.19729 and MSTRG.95524 were shown to be crucial lncRNAs. Among all the significantly changed lncRNAs identified by RT-qPCR and sequencing data, binding sites were found in four other lncRNAs (MSTRG.34610, MSTRG.10941, MSTRG.81281 and MSTRG.93731) with Aja-miR-2013-3p, a key miRNA that responds to hypoxia in sea cucumbers. The hypoxia-inducible factor (HIF-1α) was also shown as the possible targeted mRNA of Aja-miR-2013-3p. As indicated by a dual-luciferase reporter assay system, “HIF-1α gene/Aja-miR-2013-3p/MSTRG.34610” network and the “HIF-1α gene/Aja-miR-2013-3p/MSTRG.10941” network may play important roles in sea cucumbers under environmental stresses. Moreover, environmental stress altered the expression of multiple lncRNAs and mRNAs, thus affecting various biological processes in A. japonicus, including immunity, energy metabolism and the cell cycle. At the molecular level, more comprehensive responses were elicited by the combined thermal/hypoxic stress treatment than by individual stresses alone in sea cucumbers. This study lays the groundwork for future research on molecular mechanisms of echinoderm responses to thermal and hypoxic stress in the context of global climate changes. © 2019 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/158644
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, 266071, China; Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada
Recommended Citation:
Huo D.,Sun L.,Storey K.B.,et al. The regulation mechanism of lncRNAs and mRNAs in sea cucumbers under global climate changes: Defense against thermal and hypoxic stresses[J]. Science of the Total Environment,2020-01-01,709