DOI: 10.1016/j.envres.2019.108984
论文题名: The effects of glyphosate and AMPA on the mediterranean mussel Mytilus galloprovincialis and its microbiota
作者: Iori S. ; Rovere G.D. ; Ezzat L. ; Smits M. ; Ferraresso S.S. ; Babbucci M. ; Marin M.G. ; Masiero L. ; Fabrello J. ; Garro E. ; Carraro L. ; Cardazzo B. ; Patarnello T. ; Matozzo V. ; Bargelloni L. ; Milan M.
刊名: Environmental Research
ISSN: 139351
出版年: 2020
卷: 182 语种: 英语
英文关键词: AMPA
; Glyphosate
; Herbicide
; Host-microbiota interactions
; Marine species
; Transcriptional changes
; Vibrio spp.
Scopus关键词: 3 phosphoshikimate 1 carboxyvinyltransferase
; alpha amino 3 hydroxy 5 methyl 4 isoxazolepropionic acid
; aminomethylphosphonic acid
; glyphosate
; RNA 16S
; shikimic acid
; biodegradation
; bivalve
; cell component
; community care
; community composition
; ecotone
; ecotoxicology
; experimental study
; glyphosate
; homeostasis
; microbial community
; microorganism
; RNA
; toxicity
; animal experiment
; Article
; ecotoxicology
; enzymatic degradation
; gene expression
; homeostasis
; host microbe interaction
; host resistance
; intestine flora
; Mytilus galloprovincialis
; next generation sequencing
; nonhuman
; priority journal
; RNA sequence
; Vibrio
; Bacteria (microorganisms)
; Bivalvia
; Mollusca
; Mytilus galloprovincialis
; Vibrio
英文摘要: Glyphosate, the most widely used herbicide worldwide, targets the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) enzyme in the shikimate pathway found in plants and some microorganisms. While the potential for glyphosate to induce a broad range of biological effects in exposed organisms has been demonstrated, the global molecular mechanisms of toxicity and potential effects in bacterial symbionts remain unclear, in particular for ecologically important marine species such as bivalve molluscs. Here, the effects of glyphosate (GLY), its degradation product aminomethylphosphonic acid (AMPA), and a mixture of both (MIX) on the mussel M. galloprovincialis were assessed in a controlled experiment. For the first time, next generation sequencing (RNA-seq and 16S rRNA amplicon sequencing) was used to evaluate such effects at the molecular level in both the host and its respective microbiota. The results suggest that the variable capacity of bacterial species to proliferate in the presence of these compounds and the impairment of host physiological homeostasis due to AMPA and GLY toxicity may cause significant perturbations to the digestive gland microbiota, as well as elicit the spread of potential opportunistic pathogens such as Vibrio spp. The consequent host-immune system activation identified at the molecular and cellular level could be aimed at controlling changes occurring in the composition of symbiotic microbial communities. Overall, our data raise further concerns about the potential adverse effects of glyphosate and AMPA in marine species, suggesting that both the effects of direct toxicity and the ensuing changes occurring in the host-microbial community must be taken into consideration to determine the overall ecotoxicological hazard of these compounds. © 2019 Elsevier Inc. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159002
Appears in Collections: 气候变化与战略
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作者单位: Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, Legnaro (PD)35020, Italy; Department of Ecology, Evolution and Marine Biology, University of California Santa Barbara, Santa Barbara, CA 93106, United States; Department of Biology, University of Padova, Via Basssi 58/B, Padova, 35131, Italy; CONISMA – Consorzio Nazionale Interuniversitario per le Scienze del Mare, Roma, Italy
Recommended Citation:
Iori S.,Rovere G.D.,Ezzat L.,et al. The effects of glyphosate and AMPA on the mediterranean mussel Mytilus galloprovincialis and its microbiota[J]. Environmental Research,2020-01-01,182