DOI: 10.1016/j.envint.2019.105447
论文题名: Epigenetic effects of (nano)materials in environmental species – Cu case study in Enchytraeus crypticus
作者: Bicho R.C. ; Roelofs D. ; Mariën J. ; Scott-Fordsmand J.J. ; Amorim M.J.B.
刊名: Environment International
ISSN: 1604120
出版年: 2020
卷: 136 语种: 英语
英文关键词: Methylation
; Methylation sensitive high resolution melting
; Transgenerational
Scopus关键词: Alkylation
; Cell proliferation
; Chlorine compounds
; Detoxification
; DNA
; Gene encoding
; Gene expression
; Melting
; Methylation
; Nanostructured materials
; Polymerase chain reaction
; Risk assessment
; Bisulfite-sequencing
; Enchytraeus crypticus
; Environmental species
; Exposure concentration
; Gene expression analysis
; High resolution
; Histone modification
; Transgenerational
; Copper oxides
; bisulfite
; copper
; copper oxide nanoparticle
; DNA
; histone
; nanomaterial
; untranslated RNA
; annelid
; copper
; DNA
; environmental stress
; experimental study
; gene expression
; nanoparticle
; adult
; animal tissue
; Article
; bisulfite sequencing
; concentration (parameter)
; controlled study
; detoxification
; DNA methylation
; embryo
; Enchytraeus crypticus
; environmental stress
; epigenetics
; gene
; gene expression
; gene targeting
; high resolution melting analysis
; histone modification
; newborn
; nonhuman
; phenotype
; polymerase chain reaction
; priority journal
; reproduction
; Enchytraeus crypticus
英文摘要: Chemical stressors can induce epigenomic changes, i.e., changes that are transferred to the next generation, even when the stressor is removed. Literature on chemical induced epigenetic effects in environmental species is scarce. We here provide the first results on epigenetic effects caused by nanomaterials with an environmental OECD standard soil model species Enchytraeus crypticus species. We assessed the epigenetic potential in terms of global DNA methylation, gene-specific methylation via bisulfite sequencing and MS-HRM (Methylation Sensitive - High Resolution Melting), and gene expression qPCR for genes involved in DNA methylation, histone modifications, non-coding RNA and stress response mechanisms). We have exposed E. crypticus in a multigenerational (MG) test design to Cu (copper oxide nanomaterials (CuO NMs) and copper salt (CuCl2)). To link possible epigenetic effects to population changes, we used exposure concentrations (ECx) that caused a 10% and 50% reduction in the reproductive output (10% and 50% are the standards for regulatory Risk Assessment), the organisms were exposed for five consecutive generations (F1–F5) plus two generations after transferring to clean media (F5–F7), 7 generations in a total of 224 days. Results showed that MG exposure to Cu increased global DNA methylation and corresponded with phenotypic effects (reproduction). Gene expression analyses showed changes in the epigenetic, stress and detoxification gene targets, depending on the generation and Cu form, also occurring in post-exposure generations, hence indicative of transgenerational effects. There were in general clear differences between organisms exposed to different Cu-forms, hence indicate nanoparticulate-specific effects. © 2019 The Authors Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159047
Appears in Collections: 气候变化与战略
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作者单位: Departamento de Biologia & CESAM, Universidade de Aveiro, Aveiro, 3810-193, Portugal; Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, Netherlands; Department of Bioscience, Aarhus University, Vejlsovej 25, PO BOX 314, Silkeborg, DK-8600, Denmark
Recommended Citation:
Bicho R.C.,Roelofs D.,Mariën J.,et al. Epigenetic effects of (nano)materials in environmental species – Cu case study in Enchytraeus crypticus[J]. Environment International,2020-01-01,136