DOI: 10.1016/j.algal.2019.101768
论文题名: Primary metabolism is associated with the astaxanthin biosynthesis in the green algae Haematococcus pluvialis under light stress
作者: Hu C. ; Cui D. ; Sun X. ; Shi J. ; Xu N.
刊名: Algal Research
ISSN: 22119264
出版年: 2020
卷: 46 语种: 英语
英文关键词: Astaxanthin
; Glutathione metabolism
; Haematococcus pluvialis
; Metabolite profiling
; Purine and pyrimidine metabolism
; Raffinose family oligosaccharides (RFOs)
英文摘要: Haematococcus pluvialis is a kind of native bioresource, which accumulate high abundance of astaxanthin, one of the most important bioactive compounds, under various biotic and abiotic stress conditions particularly under high light. However, the global metabolic responses to high light in general and the relationship between primary metabolism and astaxanthin accumulation in particular are still obscure. In this study, non-targeted metabolomics approach was first to be used to profile the dynamic metabolic changes of H. pluvialis along astaxanthin accumulation under different light intensities, in which >200 low molecular metabolites were identified. The integration of metabolomics data with previous transcriptomic data uncovered several metabolic pathways including raffinose family oligosaccharides biosynthesis pathway, GSH metabolism pathways, pyrimidine and purine metabolism pathways, TCA cycle, and phospholipids, that are important for astaxanthin biosynthesis under high light intensity, pinpointing a metabolic shift from primary metabolism to astaxanthin biosynthesis. Exogenous application of related metabolites such as nucleotides, carbohydrates and amino acids in algal medium confirmed the metabolic link between primary metabolism and astaxanthin biosynthesis. Our results provides with a solid metabolic base for the manipulation of astaxanthin production under high light stress, and reveals metabolic insights into the adaption strategy of H. pluvialis against light stress conditions. © 2019 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159121
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: Key Laboratory of Marine Biotechnology of Zhejiang Province, Key Laboratory of Applied Marine Biotechnology of Department of Education, School of Marine Sciences, Ningbo University, Ningbo, 315800, China; Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
Recommended Citation:
Hu C.,Cui D.,Sun X.,et al. Primary metabolism is associated with the astaxanthin biosynthesis in the green algae Haematococcus pluvialis under light stress[J]. Algal Research,2020-01-01,46