DOI: 10.1016/j.ijfoodmicro.2019.108420
论文题名: Effects of temperature and water activity change on ecophysiology of ochratoxigenic Aspergillus carbonarius in field-simulating conditions
作者: Cervini C. ; Gallo A. ; Piemontese L. ; Magistà D. ; Logrieco A.F. ; Ferrara M. ; Solfrizzo M. ; Perrone G.
刊名: International Journal of Food Microbiology
ISSN: 1681605
出版年: 2020
卷: 315 语种: 英语
英文关键词: Climate change
; Gene expression
; Grape
; Growth
; Lag phase
; Ochratoxin A
Scopus关键词: basic leucine zipper transcription factor
; ochratoxin
; water
; absorption lag time
; Article
; Aspergillus carbonarius
; climate change
; drought stress
; ecophysiology
; growth rate
; mycelial growth
; nonhuman
; protein expression level
; regulator gene
; temperature
; water availability
; water stress
英文摘要: Ochratoxin A (OTA) is the primary mycotoxin threat in wine and dried vine fruits. Its presence in grape and wine is strongly related to climatic conditions and the expected climate change could represent a risk of increasing fungal colonization and OTA contamination in grapes. In this regard, the interacting effect of i) different conditions of water availability (0.93 and 0.99aw) and ii) different 10 h/14 h dark/light alternating temperature conditions simulating a nowadays (18/31 °C) and climate change scenario (20/37 °C) in high OTA risk areas of Apulia region, were studied. Lag phases prior to growth, mycelial growth rate, the expression of biosynthesis, transcription factors and regulatory genes of OTA cluster and OTA production were analysed in Aspergillus carbonarius ITEM 5010 under the combined effect of different climatic factors. At 18/31 °C and under water stress conditions (0.93 aw) the growth rate was slower than at 0.99 aw; on the contrary, at 20/37 °C a higher growth rate was observed at 0.93 aw. An over-expression of OTA genes and genes belonging to the global regulator Velvet complex was observed at 18/31 °C and 0.99 aw, with the specific OTA pathway transcription factor bZIP showing the highest expression level. The up-regulated transcription profile of the genes positively correlated with OTA production higher at 18/31 °C than at 20/37 °C and 0.99 aw; while no OTA production was detected at 0.93 aw at each of the temperature conditions tested. These findings provide preliminary evidence that the possible increase of the temperature, likely to happen in some areas of the Apulia region, may results in a reduction of both A. carbonarius spoilage and OTA production in grapes. © 2019 Elsevier B.V. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159259
Appears in Collections: 气候变化与战略
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作者单位: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Bari, Italy; Department of Economics, University of Foggia, Foggia, Italy; Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Lecce, Italy; Department of Pharmacy-Pharmaceutical Science, University of Bari “Aldo Moro”, Bari, Italy
Recommended Citation:
Cervini C.,Gallo A.,Piemontese L.,et al. Effects of temperature and water activity change on ecophysiology of ochratoxigenic Aspergillus carbonarius in field-simulating conditions[J]. International Journal of Food Microbiology,2020-01-01,315