DOI: 10.1002/ajb2.1375
论文题名: Autopolyploidy alters nodule-level interactions in the legume–rhizobium mutualism
作者: Forrester N.J. ; Ashman T.-L.
刊名: American Journal of Botany
ISSN: 29122
出版年: 2020
卷: 107, 期: 2 语种: 英语
英文关键词: Fabaceae
; Medicago
; neopolyploid
; nodule
; polyploidy
; rhizobia, Sinorhizobium
; symbiosis
Scopus关键词: legume
; mutualism
; nitrogen fixation
; nodulation
; polyploidy
; rhizobacterium
; symbiosis
; Bacteria (microorganisms)
; Fabaceae
; Medicago
; Medicago sativa subsp. caerulea
; Rhizobium
; Sinorhizobium
; Sinorhizobium medicae
; Sinorhizobium meliloti
; alfalfa
; article
; autopolyploidy
; bacterial cell
; case report
; clinical article
; confocal microscopy
; diploidy
; nitrogen fixation
; nonhuman
; plant microorganism interaction
; Sinorhizobium meliloti
; symbiosis
英文摘要: Premise: Polyploidy is a major genetic driver of ecological and evolutionary processes in plants, yet its effects on plant interactions with mutualistic microbes remain unresolved. The legume–rhizobium symbiosis regulates global nutrient cycles and plays a role in the diversification of legume species. In this mutualism, rhizobia bacteria fix nitrogen in exchange for carbon provided by legume hosts. This exchange occurs inside root nodules, which house bacterial cells and represent the interface of legume–rhizobium interactions. Although polyploidy may directly impact the legume–rhizobium mutualism, no studies have explored how it alters the internal structure of nodules. Methods: We created synthetic autotetraploids using Medicago sativa subsp. caerulea. Neotetraploid plants and their diploid progenitors were singly inoculated with two strains of rhizobia, Sinorhizobium meliloti and S. medicae. Confocal microscopy was used to quantify internal traits of nodules produced by diploid and neotetraploid plants. Results: Autotetraploid plants produced larger nodules with larger nitrogen fixation zones than diploids for both strains of rhizobia, although the significance of these differences was limited by power. Neotetraploid M. sativa subsp. caerulea plants also produced symbiosomes that were significantly larger, nearly twice the size, than those present in diploids. Conclusions: This study sheds light on how polyploidy directly affects a plant–bacterium mutualism and uncovers novel mechanisms. Changes in plant–microbe interactions that directly result from polyploidy likely contribute to the increased ability of polyploid legumes to establish in diverse environments. © 2019 Botanical Society of America Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159928
Appears in Collections: 气候变化与战略
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作者单位: Department of Biological Sciences, University of Pittsburgh, 4249 Fifth Avenue, Pittsburgh, PA 15260, United States
Recommended Citation:
Forrester N.J.,Ashman T.-L.. Autopolyploidy alters nodule-level interactions in the legume–rhizobium mutualism[J]. American Journal of Botany,2020-01-01,107(2)