globalchange  > 气候变化与战略
DOI: 10.1007/s00338-019-01875-9
论文题名:
Development of a multi-assay approach for monitoring coral diversity using eDNA metabarcoding
作者: Alexander J.B.; Bunce M.; White N.; Wilkinson S.P.; Adam A.A.S.; Berry T.; Stat M.; Thomas L.; Newman S.J.; Dugal L.; Richards Z.T.
刊名: Coral Reefs
ISSN: 7224028
出版年: 2020
卷: 39, 期:1
语种: 英语
英文关键词: Biodiversity ; Biomonitoring ; Coral reefs ; Environmental DNA ; Scleractinia
Scopus关键词: anthropogenic effect ; community composition ; coral reef ; DNA ; genetic variation ; phylogenetics ; species diversity ; surface water ; taxonomy ; Anthozoa ; Otus ; Porifera ; Scleractinia
英文摘要: Cumulative anthropogenic pressures have triggered a global decline in the health of marine ecosystems, and coral reefs, in particular, are in crisis. With climate and population-related pressures predicted to intensify in the coming decades, it is increasingly crucial to develop cost-effective and accurate monitoring tools to document changes to these important ecosystems. Environmental DNA (eDNA) coupled with metabarcoding is a powerful tool for surveying a wide variety of biota. Here, we develop a baseline eDNA toolkit targeting scleractinian corals and validate its performance in conjunction with data derived on traditional diver-based visual surveys at the Cocos (Keeling) Islands. Three assays targeting the ITS2 and 16S barcoding regions were designed, which broadly detected diversity within Scleractinia and Porifera. Our eDNA assays recovered 78 ITS2 operational taxonomic units (OTUs) from 25 scleractinian genera which is comparable to the level of diversity recorded on visual surveys (68 species from 26 genera). There were some notable differences in the species detected using eDNA versus visual records that may relate to either misidentifications, intragenic variation, differential assay performance or cryptic species. Our data demonstrate that a multi-assay eDNA analytical approach, applied on surface water collections, represents a powerful and complementary way to survey diversity that can also reveal fine scale spatial differentiation in community composition. With further refinement and improved reference databases, we envisage eDNA to become a powerful complement to visual surveys and to play a key role in monitoring the health and diversity of complex coral reefs ecosystems. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/159932
Appears in Collections:气候变化与战略

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作者单位: Coral Conservation and Research Group, Trace and Environmental DNA (TrEnD) Laboratory, School of Molecular and Life Sciences, Curtin University, Bentley, WA 6102, Australia; Environmental Protection Authority, 215 Lambton Quay, Wellington, 6011, New Zealand; School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia; Australian Institute of Marine Science and the UWA Oceans Institute, Indian Ocean Marine Research Centre, The University of Western Australia, Crawley, WA 6009, Australia; Western Australian Fisheries and Marine Research Laboratories, Department of Primary Industries and Regional Development, Government of Western Australia, PO Box 20, North Beach, WA 6920, Australia; Aquatic Zoology Department, Western Australian Museum, Welshpool, WA 6106, Australia

Recommended Citation:
Alexander J.B.,Bunce M.,White N.,et al. Development of a multi-assay approach for monitoring coral diversity using eDNA metabarcoding[J]. Coral Reefs,2020-01-01,39(1)
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