globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1802940115
论文题名:
Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA
作者: Welkie D.G.; Rubin B.E.; Chang Y.-G.; Diamond S.; Rifkin S.A.; LiWang A.; Golden S.S.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:30
起始页码: E7174
结束页码: E7183
语种: 英语
英文关键词: Circadian clock ; Cyanobacteria ; Diurnal physiology ; Photosynthesis ; Transposon sequencing
Scopus关键词: clock protein KaiA ; transcription factor CLOCK ; unclassified drug ; bacterial protein ; circadian rhythm signaling protein ; KaiA protein, cyanobacteria ; Article ; bacterial gene ; bacterial viability ; circadian rhythm ; controlled study ; cyanophage ; gene identification ; gene library ; gene loss ; gene mutation ; genetic screening ; genome-wide association study ; kaiA gene ; light dark cycle ; metabolic regulation ; molecular dynamics ; nonhuman ; phototrophic bacterium ; priority journal ; protein interaction ; survival ; Synechococcus elongatus ; transposon ; circadian rhythm ; genetics ; metabolism ; photosynthesis ; physiology ; signal transduction ; Synechococcus ; Bacterial Proteins ; Circadian Clocks ; Circadian Rhythm Signaling Peptides and Proteins ; Genome-Wide Association Study ; Photosynthesis ; Signal Transduction ; Synechococcus
英文摘要: The recurrent pattern of light and darkness generated by Earth’s axial rotation has profoundly influenced the evolution of organisms, selecting for both biological mechanisms that respond acutely to environmental changes and circadian clocks that program physiology in anticipation of daily variations. The necessity to integrate environmental responsiveness and circadian programming is exemplified in photosynthetic organisms such as cyanobacteria, which depend on light-driven photochemical processes. The cyanobacterium Synechococcus elongatus PCC 7942 is an excellent model system for dissecting these entwined mechanisms. Its core circadian oscillator, consisting of three proteins, KaiA, KaiB, and KaiC, transmits time-of-day signals to clock-output proteins, which reciprocally regulate global transcription. Research performed under constant light facilitates analysis of intrinsic cycles separately from direct environmental responses but does not provide insight into how these regulatory systems are integrated during light–dark cycles. Thus, we sought to identify genes that are specifically necessary in a day–night environment. We screened a dense bar-coded transposon library in both continuous light and daily cycling conditions and compared the fitness consequences of loss of each nonessential gene in the genome. Although the clock itself is not essential for viability in light–dark cycles, the most detrimental mutations revealed by the screen were those that disrupt KaiA. The screen broadened our understanding of light–dark survival in photosynthetic organisms, identified unforeseen clock–protein interaction dynamics, and reinforced the role of the clock as a negative regulator of a nighttime metabolic program that is essential for S. elongatus to survive in the dark. © 2018 National Academy of Sciences. All Rights Reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/162188
Appears in Collections:气候变化与战略

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作者单位: Welkie, D.G., Center for Circadian Biology, University of California, San Diego, La Jolla, CA 92093, United States; Rubin, B.E., Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, United States; Chang, Y.-G., School of Natural Sciences, University of California, Merced, CA 95343, United States; Diamond, S., Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, United States, Department of Earth and Planetary Science, University of California, Berkeley, CA 94720, United States; Rifkin, S.A., Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, United States; LiWang, A., Center for Circadian Biology, University of California, San Diego, La Jolla, CA 92093, United States, School of Natural Sciences, University of California, Merced, CA 95343, United States, Chemistry and Chemical Biology, University of California, Merced, CA 95343, United States, Quantitative and Systems Biology, University of California, Merced, CA 95343, United States, Health Sciences Research Institute, University of California, Merced, CA 95343, United States, Center for Cellular and Biomolecular Machines, University of California, Merced, CA 95343, United States; Golden, S.S., Center for Circadian Biology, University of California, San Diego, La Jolla, CA 92093, United States, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, United States

Recommended Citation:
Welkie D.G.,Rubin B.E.,Chang Y.-G.,et al. Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(30)
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