globalchange  > 气候变化与战略
DOI: 10.1073/pnas.0706787104
论文题名:
Ancient bacteria show evidence of DNA repair
作者: Johnson S.S.; Hebsgaard M.B.; Christensen T.R.; Mastepanov M.; Nielsen R.; Munch K.; Brand T.; Gilbert M.T.P.; Zuber M.T.; Bunce M.; Rønn R.; Gilichinsky D.; Froese D.; Willerslev E.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2007
卷: 104, 期:36
起始页码: 14401
结束页码: 14405
语种: 英语
英文关键词: DNA damage ; Long-term microbial survival ; Metabolic activity
Scopus关键词: bacterial DNA ; carbon dioxide ; nucleotide ; ribosome DNA ; article ; bacterial cell ; bacterial metabolism ; bacterium ; cell survival ; cell viability ; DNA damage ; DNA repair ; DNA sequence ; molecular cloning ; nonhuman ; nucleotide sequence ; polymerase chain reaction ; priority journal ; unindexed sequence ; bacterium ; gene amplification ; genetics ; microbiology ; molecular genetics ; nucleotide sequence ; Bacteria (microorganisms) ; Bacteria ; Base Sequence ; DNA Repair ; DNA, Bacterial ; Gene Amplification ; Molecular Sequence Data ; Soil Microbiology
英文摘要: Recent claims of cultivable ancient bacteria within sealed environments highlight our limited understanding of the mechanisms behind long-term cell survival. It remains unclear how dormancy, a favored explanation for extended cellular persistence, can cope with spontaneous genomic decay over geological timescales. There has been no direct evidence in ancient microbes for the most likely mechanism, active DNA repair, or for the metabolic activity necessary to sustain it. In this paper, we couple PCR and enzymatic treatment of DNA with direct respiration measurements to investigate long-term survival of bacteria sealed in frozen conditions for up to one million years. Our results show evidence of bacterial survival in samples up to half a million years in age, making this the oldest independently authenticated DNA to date obtained from viable cells. Additionally, we find strong evidence that this long-term survival is closely tied to cellular metabolic activity and DNA repair that over time proves to be superior to dormancy as a mechanism in sustaining bacteria viability. © 2007 by The National Academy of Sciences of the USA.
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/162237
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Johnson, S.S., Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 54-810, Cambridge, MA 02139, United States, Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Hebsgaard, M.B., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Christensen, T.R., GeoBiosphere Science Centre, Lund University, Sölvegatan 12, Lund, 22362, Sweden; Mastepanov, M., GeoBiosphere Science Centre, Lund University, Sölvegatan 12, Lund, 22362, Sweden; Nielsen, R., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Munch, K., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Brand, T., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Gilbert, M.T.P., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Zuber, M.T., Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology, 77 Massachusetts Avenue, 54-810, Cambridge, MA 02139, United States; Bunce, M., Ancient DNA Research Laboratory, Murdoch University, Perth, WA 6150, Australia; Rønn, R., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark; Gilichinsky, D., Soil Cryology Laboratory, Institute of Physicochemical and Biological Problems in Soil Science, Russian Academy of Sciences, Pushchino, Russian Federation; Froese, D., Department of Earth and Atmospheric Sciences, University of Alberta, Edmonton, Alta. T6G283, Canada; Willerslev, E., Centre for Ancient Genetics, Institute of Biology, University of Copenhagen, Universitetsparken 15, DK-2100 Copenhagen, Denmark

Recommended Citation:
Johnson S.S.,Hebsgaard M.B.,Christensen T.R.,et al. Ancient bacteria show evidence of DNA repair[J]. Proceedings of the National Academy of Sciences of the United States of America,2007-01-01,104(36)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Johnson S.S.]'s Articles
[Hebsgaard M.B.]'s Articles
[Christensen T.R.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Johnson S.S.]'s Articles
[Hebsgaard M.B.]'s Articles
[Christensen T.R.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Johnson S.S.]‘s Articles
[Hebsgaard M.B.]‘s Articles
[Christensen T.R.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.