globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2013161117
论文题名:
EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity
作者: Acheampong A.K.; Shanks C.; Cheng C.-Y.; Eric Schaller G.; Dagdas Y.; Kieber J.J.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:43
起始页码: 27034
结束页码: 27043
语种: 英语
英文关键词: Carbon starvation ; Concanamycin A ; Cytokinin signaling ; Selective autophagy
英文摘要: The phytohormone cytokinin influences many aspects of plant growth and development, several of which also involve the cellular process of autophagy, including leaf senescence, nutrient remobilization, and developmental transitions. The Arabidopsis type-A response regulators (type-A ARR) are negative regulators of cytokinin signaling that are transcriptionally induced in response to cytokinin. Here, we describe a mechanistic link between cytokinin signaling and autophagy, demonstrating that plants modulate cytokinin sensitivity through autophagic regulation of type-A ARR proteins. Type-A ARR proteins were degraded by autophagy in an AUTOPHAGY-RELATED (ATG)5-dependent manner, and this degradation is promoted by phosphorylation on a conserved aspartate in the receiver domain of the type-A ARRs. EXO70D family members interacted with type-A ARR proteins, likely in a phosphorylation-dependent manner, and recruited them to autophagosomes via interaction of the EXO70D AIM with the core autophagy protein, ATG8. Consistently, loss-of-function exo70D1,2,3 mutants exhibited compromised targeting of type-A ARRs to autophagic vesicles, have elevated levels of type-A ARR proteins, and are hyposensitive to cytokinin. Disruption of both type-A ARRs and EXO70D1,2,3 compromised survival in carbon-deficient conditions, suggesting interaction between autophagy and cytokinin responsiveness in response to stress. These results indicate that the EXO70D proteins act as selective autophagy receptors to target type-A ARR cargos for autophagic degradation, demonstrating modulation of cytokinin signaling by selective autophagy. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163373
Appears in Collections:气候变化与战略

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作者单位: Acheampong, A.K., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Shanks, C., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Cheng, C.-Y., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States; Eric Schaller, G., Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, United States; Dagdas, Y., Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, 1030, Austria; Kieber, J.J., Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States

Recommended Citation:
Acheampong A.K.,Shanks C.,Cheng C.-Y.,et al. EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(43)
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