globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2014371117
论文题名:
Segregation of four Agrobacterium tumefaciens replicons during polar growth: PopZ and PodJ control segregation of essential replicons
作者: Robalino-Espinosa J.S.; Zupan J.R.; Chavez-Arroyo A.; Zambryski P.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:42
起始页码: 26366
结束页码: 26373
语种: 英语
英文关键词: Agrobacterium tumefaciens ; Multipartite genomes ; Polar growth ; PopZ and PodJ ; Replication and segregation
Scopus关键词: article ; cell cycle ; chromosome ; replicon ; Ti plasmid (plant)
英文摘要: Agrobacterium tumefaciens C58 contains four replicons, circular chromosome (CC), linear chromosome (LC), cryptic plasmid (pAt), and tumor-inducing plasmid (pTi), and grows by polar growth from a single growth pole (GP), while the old cell compartment and its old pole (OP) do not elongate. We monitored the replication and segregation of these four genetic elements during polar growth. The three largest replicons (CC, LC, pAt) reside in the OP compartment prior to replication; post replication one copy migrates to the GP prior to division. CC resides at a fixed location at the OP and replicates first. LC does not stay fixed at the OP once the cell cycle begins and replicates from varied locations 20 min later than CC. pAt localizes similarly to LC prior to replication, but replicates before the LC and after the CC. pTi does not have a fixed location, and post replication it segregates randomly throughout old and new cell compartments, while undergoing one to three rounds of replication during a single cell cycle. Segregation of the CC and LC is dependent on the GP and OP identity factors PopZ and PodJ, respectively. Without PopZ, replicated CC and LC do not efficiently partition, resulting in sibling cells without CC or LC. Without PodJ, the CC and LC exhibit abnormal localization to the GP at the beginning of the cell cycle and replicate from this position. These data reveal PodJ plays an essential role in CC and LC tethering to the OP during early stages of polar growth. © 2020 National Academy of Sciences. All rights reserved.
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被引频次[WOS]:9   [查看WOS记录]     [查看WOS中相关记录]
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163375
Appears in Collections:气候变化与战略

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作者单位: Robalino-Espinosa, J.S., Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States, Department of Molecular and Cell Biology, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, Netherlands; Zupan, J.R., Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; Chavez-Arroyo, A., Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; Zambryski, P., Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States

Recommended Citation:
Robalino-Espinosa J.S.,Zupan J.R.,Chavez-Arroyo A.,et al. Segregation of four Agrobacterium tumefaciens replicons during polar growth: PopZ and PodJ control segregation of essential replicons[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(42)
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