globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2005600117
论文题名:
The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets in Chlamydomonas reinhardtii
作者: Lee J.; Yamaoka Y.; Kong F.; Cagnon C.; Beyly-Adriano A.; Jang S.; Gao P.; Kang B.-H.; Li-Beisson Y.; Lee Y.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:37
起始页码: 23131
结束页码: 23139
语种: 英语
Scopus关键词: apolipoprotein A1 ; apolipoprotein E ; binding protein ; DTH1 protein ; fat droplet ; phosphatidylethanolamine ; recombinant protein ; triacylglycerol ; unclassified drug ; algal protein ; fat droplet ; nitrogen ; phosphatidylethanolamine binding protein ; phospholipid ; triacylglycerol ; algal growth ; alpha helix ; Article ; Chlamydomonas reinhardtii ; controlled study ; dth1 1 gene ; dth1 2 gene ; dth1 3 gene ; DTH1 gene ; gene ; gene overexpression ; genetic transcription ; lipid metabolism ; loss of function mutation ; mutant ; nonhuman ; photosynthesis ; priority journal ; protein binding ; protein domain ; protein function ; protein localization ; amino acid sequence ; Chlamydomonas reinhardtii ; lipid metabolism ; metabolism ; physiology ; Algal Proteins ; Amino Acid Sequence ; Chlamydomonas reinhardtii ; Lipid Droplets ; Lipid Metabolism ; Nitrogen ; Phosphatidylethanolamine Binding Protein ; Phospholipids ; Photosynthesis ; Triglycerides
英文摘要: Lipid droplets (LDs) are intracellular organelles found in a wide range of organisms and play important roles in stress tolerance. During nitrogen (N) starvation, Chlamydomonas reinhardtii stores large amounts of triacylglycerols (TAGs) inside LDs. When N is resupplied, the LDs disappear and the TAGs are degraded, presumably providing carbon and energy for regrowth. The mechanism by which cells degrade LDs is poorly understood. Here, we isolated a mutant (dth1-1, Delayed in TAG Hydrolysis 1) in which TAG degradation during recovery from N starvation was compromised. Consequently, the dth1-1 mutant grew poorly compared to its parental line during N recovery. Two additional independent loss-of-function mutants (dth1-2 and dth1-3) also exhibited delayed TAG remobilization. DTH1 transcript levels increased sevenfold upon N resupply, and DTH1 protein was localized to LDs. DTH1 contains a putative lipid-binding domain (DTH1LBD) with alpha helices predicted to be structurally similar to those in apolipoproteins E and A–I. Recombinant DTH1LBD bound specifically to phosphatidylethanolamine (PE), a major phospholipid coating the LD surface. Overexpression of DTH1LBD in Chlamydomonas phenocopied the dth1 mutant’s defective TAG degradation, suggesting that the function of DTH1 depends on its ability to bind PE. Together, our results demonstrate that the lipid-binding DTH1 plays an essential role in LD degradation and provide insight into the molecular mechanism of protein anchorage to LDs at the LD surface in photosynthetic cells. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163393
Appears in Collections:气候变化与战略

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作者单位: Lee, J., Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang, 37673, South Korea; Yamaoka, Y., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea; Kong, F., Laboratory of Marine Biotechnology, School of Bioengineering, Dalian University of Technology, Dalian, 116024, China; Cagnon, C., Aix-Marseille University, Commission for Atomic Energy and Alternative Energies (CEA), CNRS, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), CEA Cadarache, Saint Paul-Lez-Durance, 13108, France; Beyly-Adriano, A., Aix-Marseille University, Commission for Atomic Energy and Alternative Energies (CEA), CNRS, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), CEA Cadarache, Saint Paul-Lez-Durance, 13108, France; Jang, S., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea; Gao, P., State Key Laboratory of Agrobiotechnology, School of Life Sciences, Chinese University of Hong Kong, Hong Kong New Territories, 999077, Hong Kong; Kang, B.-H., State Key Laboratory of Agrobiotechnology, School of Life Sciences, Chinese University of Hong Kong, Hong Kong New Territories, 999077, Hong Kong; Li-Beisson, Y., Aix-Marseille University, Commission for Atomic Energy and Alternative Energies (CEA), CNRS, Institut de Biosciences et Biotechnologies Aix-Marseille (BIAM), CEA Cadarache, Saint Paul-Lez-Durance, 13108, France; Lee, Y., Department of Life Sciences, Pohang University of Science and Technology, Pohang, 37673, South Korea

Recommended Citation:
Lee J.,Yamaoka Y.,Kong F.,et al. The phosphatidylethanolamine-binding protein DTH1 mediates degradation of lipid droplets in Chlamydomonas reinhardtii[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(37)
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