DOI: 10.1073/pnas.1920337117
论文题名: Cleavage-furrow formation without F-actin in Chlamydomonas
作者: Onishi M. ; Umen J.G. ; Cross F.R. ; Pringle J.R.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期: 31 起始页码: 18511
结束页码: 18520
语种: 英语
英文关键词: Cell division
; Chloroplast division
; Cytokinesis
; Microtubules
; Myosin
Scopus关键词: Article
; cell membrane
; Chlamydomonas reinhardtii
; chloroplast
; controlled study
; cytokinesis
; depolymerization
; Dictyostelium
; eukaryote
; microtubule
; Naegleria gruberi
; nonhuman
; phragmoplast
; priority journal
; protein analysis
; protein cleavage
; protein function
; protein localization
; Saccharomyces cerevisiae
; actin filament
; cell division
; chemistry
; Chlamydomonas
; cytology
; metabolism
; actin
; myosin
; protein binding
; Actin Cytoskeleton
; Actins
; Cell Division
; Chlamydomonas
; Cytokinesis
; Microtubules
; Myosins
; Protein Binding
英文摘要: It is widely believed that cleavage-furrow formation during cytokinesis is driven by the contraction of a ring containing F-actin and type-II myosin. However, even in cells that have such rings, they are not always essential for furrow formation. Moreover, many taxonomically diverse eukaryotic cells divide by furrowing but have no type-II myosin, making it unlikely that an actomyosin ring drives furrowing. To explore this issue further, we have used one such organism, the green alga Chlamydomonas reinhardtii. We found that although F-actin is associated with the furrow region, none of the three myosins (of types VIII and XI) is localized there. Moreover, when F-actin was eliminated through a combination of a mutation and a drug, furrows still formed and the cells divided, although somewhat less efficiently than normal. Unexpectedly, division of the large Chlamydomonas chloroplast was delayed in the cells lacking F-actin; as this organelle lies directly in the path of the cleavage furrow, this delay may explain, at least in part, the delay in cytokinesis itself. Earlier studies had shown an association of microtubules with the cleavage furrow, and we used a fluorescently tagged EB1 protein to show that microtubules are still associated with the furrows in the absence of F-actin, consistent with the possibility that the microtubules are important for furrow formation. We suggest that the actomyosin ring evolved as one way to improve the efficiency of a core process for furrow formation that was already present in ancestral eukaryotes. © 2020 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163416
Appears in Collections: 气候变化与战略
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作者单位: Onishi, M., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States, Department of Biology, Duke University, Durham, NC 27708, United States; Umen, J.G., Donald Danforth Plant Science Center, St. Louis, MO 63132, United States; Cross, F.R., Rockefeller University, New York, NY 10065, United States; Pringle, J.R., Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, United States
Recommended Citation:
Onishi M.,Umen J.G.,Cross F.R.,et al. Cleavage-furrow formation without F-actin in Chlamydomonas[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(31)