globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1920291117
论文题名:
Surface sensing stimulates cellular differentiation in Caulobacter crescentus
作者: Snyder R.A.; Ellison C.K.; Severin G.B.; Whitfield G.B.; Waters C.M.; Brun Y.V.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:30
起始页码: 17984
结束页码: 17991
语种: 英语
英文关键词: Bacteria ; Cell cycle ; Cellular differentiation ; Surface sensing ; Type IV pili
Scopus关键词: protein histidine kinase ; secretin ; secretin CpaC ; unclassified drug ; bis(3',5')-cyclic diguanylic acid ; cyclic GMP ; Article ; bacterium pilus ; Caulobacter vibrioides ; cell cycle progression ; cell differentiation ; cell fate ; chromosome replication ; controlled study ; DNA replication ; enzyme activity ; enzyme localization ; nonhuman ; priority journal ; bacterial phenomena and functions ; biological model ; Caulobacter vibrioides ; cell cycle ; fimbria ; Gram negative infection ; metabolism ; microbiology ; mutation ; physiology ; Bacterial Physiological Phenomena ; Caulobacter crescentus ; Cell Cycle ; Cyclic GMP ; DNA Replication ; Fimbriae, Bacterial ; Gram-Negative Bacterial Infections ; Models, Biological ; Mutation
英文摘要: Cellular differentiation is a fundamental strategy used by cells to generate specialized functions at specific stages of development. The bacterium Caulobacter crescentus employs a specialized dimorphic life cycle consisting of two differentiated cell types. How environmental cues, including mechanical inputs such as contact with a surface, regulate this cell cycle remain unclear. Here, we find that surface sensing by the physical perturbation of retracting extracellular pilus filaments accelerates cell-cycle progression and cellular differentiation. We show that physical obstruction of dynamic pilus activity by chemical perturbation or by a mutation in the outer-membrane pilus secretin CpaC stimulates early initiation of chromosome replication. In addition, we find that surface contact stimulates cell-cycle progression by demonstrating that surface-stimulated cells initiate early chromosome replication to the same extent as planktonic cells with obstructed pilus activity. Finally, we show that obstruction of pilus retraction stimulates the synthesis of the cell-cycle regulator cyclic diguanylate monophosphate (c-di-GMP) through changes in the activity and localization of two key regulatory histidine kinases that control cell fate and differentiation. Together, these results demonstrate that surface contact and sensing by alterations in pilus activity stimulate C. crescentus to bypass its developmentally programmed temporal delay in cell differentiation to more quickly adapt to a surface-associated lifestyle. © 2020 National Academy of Sciences. All rights reserved.
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163423
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Snyder, R.A., Department of Biology, Indiana University, Bloomington, IN 47405, United States; Ellison, C.K., Department of Biology, Indiana University, Bloomington, IN 47405, United States; Severin, G.B., Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48823, United States; Whitfield, G.B., Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H3C 3J7, Canada; Waters, C.M., Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48823, United States; Brun, Y.V., Department of Biology, Indiana University, Bloomington, IN 47405, United States, Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montréal, QC H3C 3J7, Canada

Recommended Citation:
Snyder R.A.,Ellison C.K.,Severin G.B.,et al. Surface sensing stimulates cellular differentiation in Caulobacter crescentus[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(30)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Snyder R.A.]'s Articles
[Ellison C.K.]'s Articles
[Severin G.B.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Snyder R.A.]'s Articles
[Ellison C.K.]'s Articles
[Severin G.B.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Snyder R.A.]‘s Articles
[Ellison C.K.]‘s Articles
[Severin G.B.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.