DOI: 10.1073/pnas.1922365117
论文题名: Phase transition of RNA−protein complexes into ordered hollow condensates
作者: Alshareedah I. ; Moosa M.M. ; Raju M. ; Potoyan D.A. ; Banerjee P.R.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期: 27 起始页码: 15650
结束页码: 15658
语种: 英语
英文关键词: Biomolecular condensates
; MD simulation
; Nucleoprotein assembly
; Optical tweezer
; RNA vesicles
Scopus关键词: arginine
; lipid
; lysine
; nucleoprotein
; polypeptide
; intrinsically disordered protein
; multiprotein complex
; RNA
; Article
; complex formation
; cross linking
; dissolution
; encapsulation
; hydrophobicity
; molecular weight
; phase transition
; physical chemistry
; priority journal
; protein RNA binding
; protein structure
; static electricity
; anisotropy
; chemistry
; genetics
; optical tweezers
; phase transition
; Anisotropy
; Intrinsically Disordered Proteins
; Lipids
; Multiprotein Complexes
; Nucleoproteins
; Optical Tweezers
; Phase Transition
; RNA
英文摘要: Liquid−liquid phase separation of multivalent intrinsically disordered protein−RNA complexes is ubiquitous in both natural and biomimetic systems. So far, isotropic liquid droplets are the most commonly observed topology of RNA−protein condensates in experiments and simulations. Here, by systematically studying the phase behavior of RNA−protein complexes across varied mixture compositions, we report a hollow vesicle-like condensate phase of nucleoprotein assemblies that is distinct from RNA−protein droplets. We show that these vesicular condensates are stable at specific mixture compositions and concentration regimes within the phase diagram and are formed through the phase separation of anisotropic protein−RNA complexes. Similar to membranes composed of amphiphilic lipids, these nucleoprotein−RNA vesicular membranes exhibit local ordering, size-dependent permeability, and selective encapsulation capacity without sacrificing their dynamic formation and dissolution in response to physicochemical stimuli. Our findings suggest that protein−RNA complexes can robustly create lipid-free vesicle-like enclosures by phase separation. © 2020 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163443
Appears in Collections: 气候变化与战略
There are no files associated with this item.
作者单位: Alshareedah, I., Department of Physics, University at Buffalo, State University of New York, Buffalo, NY 14260, United States; Moosa, M.M., Department of Physics, University at Buffalo, State University of New York, Buffalo, NY 14260, United States; Raju, M., Department of Chemistry, Iowa State University, Ames, IA 50011, United States; Potoyan, D.A., Department of Chemistry, Iowa State University, Ames, IA 50011, United States; Banerjee, P.R., Department of Physics, University at Buffalo, State University of New York, Buffalo, NY 14260, United States
Recommended Citation:
Alshareedah I.,Moosa M.M.,Raju M.,et al. Phase transition of RNA−protein complexes into ordered hollow condensates[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(27)