globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1909306116
论文题名:
GDGT cyclization proteins identify the dominant archaeal sources of tetraether lipids in the ocean
作者: Zeng Z.; Liu X.-L.; Farley K.R.; Wei J.H.; Metcalf W.W.; Summons R.E.; Welander P.V.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:45
起始页码: 22505
结束页码: 22511
语种: 英语
英文关键词: GDGT ; Paleotemperature proxies ; Radical SAM ; Sulfolobus
Scopus关键词: cyclopentane ; glycerol dibiphytanyl glycerol tetraether derivative ; GrsA protein ; GrsB protein ; membrane lipid ; s adenosylmethionine ; synthetase ; unclassified drug ; archaeon ; Article ; biochemical analysis ; bioinformatics ; comparative genomic hybridization ; controlled study ; cyclization ; environmental factor ; environmental temperature ; Euryarchaeota ; gene deletion ; lipid analysis ; nonhuman ; ocean environment ; phylogeny ; priority journal ; protein depletion ; protein synthesis ; sequence homology ; Sulfolobus acidocaldarius ; Thaumarchaeota ; thermoacidophilic archaeon
英文摘要: Glycerol dibiphytanyl glycerol tetraethers (GDGTs) are distinctive archaeal membrane-spanning lipids with up to eight cyclopentane rings and/or one cyclohexane ring. The number of rings added to the GDGT core structure can vary as a function of environmental conditions, such as changes in growth temperature. This physiological response enables cyclic GDGTs preserved in sediments to be employed as proxies for reconstructing past global and regional temperatures and to provide fundamental insights into ancient climate variability. Yet, confidence in GDGT-based paleotemperature proxies is hindered by uncertainty concerning the archaeal communities contributing to GDGT pools in modern environments and ambiguity in the environmental and physiological factors that affect GDGT cyclization in extant archaea. To properly constrain these uncertainties, a comprehensive understanding of GDGT biosynthesis is required. Here, we identify 2 GDGT ring synthases, GrsA and GrsB, essential for GDGT ring formation in Sulfolobus acidocaldarius. Both proteins are radical S-adenosylmethionine proteins, indicating that GDGT cyclization occurs through a free radical mechanism. In addition, we demonstrate that GrsA introduces rings specifically at the C-7 position of the core GDGT lipid, while GrsB cyclizes at the C-3 position, suggesting that cyclization patterns are differentially controlled by 2 separate enzymes and potentially influenced by distinct environmental factors. Finally, phylogenetic analyses of the Grs proteins reveal that marine Thaumarchaeota, and not Euryarchaeota, are the dominant source of cyclized GDGTs in open ocean settings, addressing a major source of uncertainty in GDGT-based paleotemperature proxy applications. © 2019 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163519
Appears in Collections:气候变化与战略

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作者单位: Zeng, Z., Department of Earth System Science, Stanford University, Stanford, CA 94305, United States; Liu, X.-L., Department of Geosciences, University of Oklahoma, Norman, OK 73019, United States; Farley, K.R., Department of Microbiology, University of Illinois Urbana–Champaign, Urbana, IL 61801, United States; Wei, J.H., Department of Earth System Science, Stanford University, Stanford, CA 94305, United States; Metcalf, W.W., Department of Microbiology, University of Illinois Urbana–Champaign, Urbana, IL 61801, United States, Institute for Genomic Biology, University of Illinois Urbana–Champaign, Urbana, IL 61801, United States; Summons, R.E., Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, United States; Welander, P.V., Department of Earth System Science, Stanford University, Stanford, CA 94305, United States

Recommended Citation:
Zeng Z.,Liu X.-L.,Farley K.R.,et al. GDGT cyclization proteins identify the dominant archaeal sources of tetraether lipids in the ocean[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(45)
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