DOI: 10.1073/pnas.1906119116
论文题名: Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation
作者: Zhou X. ; Wang J. ; Herrmann J. ; Moerner W.E. ; Shapiro L.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期: 31 起始页码: 15661
结束页码: 15670
语种: 英语
英文关键词: DNA methyltransferase
; Epigenetics
; Lon protease
; Protein sequestration
Scopus关键词: bacterial DNA
; DNA methyltransferase
; endopeptidase La
; bacterial DNA
; bacterial protein
; Article
; asymmetric cell division
; bacterial cell
; bacterial chromosome
; Caulobacter vibrioides
; cell cycle progression
; cell cycle regulation
; cell differentiation
; controlled study
; DNA methylation
; DNA replication
; enzyme degradation
; nonhuman
; priority journal
; progeny
; protein degradation
; single molecule imaging
; transcription initiation
; bacterial chromosome
; biosynthesis
; Caulobacter
; cell cycle
; DNA replication
; genetics
; metabolism
; Bacterial Proteins
; Caulobacter
; Cell Cycle
; Chromosomes, Bacterial
; DNA Methylation
; DNA Replication
; DNA, Bacterial
英文摘要: The cell cycle-regulated methylation state of Caulobacter DNA mediates the temporal control of transcriptional activation of several key regulatory proteins. Temporally controlled synthesis of the CcrM DNA methyltransferase and Lon-mediated proteolysis restrict CcrM to a specific time in the cell cycle, thereby allowing the maintenance of the hemimethylated state of the chromosome during the progression of DNA replication. We determined that a chromosomal DNA-based platform stimulates CcrM degradation by Lon and that the CcrM C terminus both binds to its DNA substrate and is recognized by the Lon protease. Upon asymmetric cell division, swarmer and stalked progeny cells employ distinct mechanisms to control active CcrM. In progeny swarmer cells, CcrM is completely degraded by Lon before its differentiation into a replication-competent stalked cell later in the cell cycle. In progeny stalked cells, however, accumulated CcrM that has not been degraded before the immediate initiation of DNA replication is sequestered to the cell pole. Single-molecule imaging demonstrated physical anticorrelation between sequestered CcrM and chromosomal DNA, thus preventing DNA remethylation. The distinct control of available CcrM in progeny swarmer and stalked cells serves to protect the hemimethylated state of DNA during chromosome replication, enabling robustness of cell cycle progression. © 2019 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163544
Appears in Collections: 气候变化与战略
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作者单位: Zhou, X., Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States; Wang, J., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Herrmann, J., Department of Structural Biology, Stanford University, Stanford, CA 94305, United States; Moerner, W.E., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Shapiro, L., Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States, Chan Zuckerberg Biohub, San Francisco, CA 94158, United States
Recommended Citation:
Zhou X.,Wang J.,Herrmann J.,et al. Asymmetric division yields progeny cells with distinct modes of regulating cell cycle-dependent chromosome methylation[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(31)