DOI: 10.1073/pnas.1812351116
论文题名: Exploring fast proton transfer events associated with lateral proton diffusion on the surface of membranes
作者: Amdursky N. ; Lin Y. ; Aho N. ; Groenhof G.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期: 7 起始页码: 2443
结束页码: 2451
语种: 英语
英文关键词: Excited-state proton transfer
; Lipid vesicles
; Molecular dynamics
; Photoacid
; Proton diffusion
Scopus关键词: ampholyte
; lipid
; phosphate
; Article
; biological model
; biomembrane
; chemical parameters
; fast proton transfer event
; kinetics
; light exposure
; lipid vesicle
; mathematical model
; membrane transport
; molecular probe
; pH
; priority journal
; proton transport
; surface property
英文摘要: Proton diffusion (PD) across biological membranes is a fundamental process in many biological systems, and much experimental and theoretical effort has been employed for deciphering it. Here, we report on a spectroscopic probe, which can be tightly tethered to the membrane, for following fast (nanosecond) proton transfer events on the surface of membranes. Our probe is composed of a photoacid that serves as our light-induced proton source for the initiation of the PD process. We use our probe to follow PD, and its pH dependence, on the surface of lipid vesicles composed of a zwitterionic headgroup, a negative headgroup, a headgroup that is composed only from the negative phosphate group, or a positive headgroup without the phosphate group. We reveal that the PD kinetic parameters are highly sensitive to the nature of the lipid headgroup, ranging from a fast lateral diffusion at some membranes to the escape of protons from surface to bulk (and vice versa) at others. By referring to existing theoretical models for membrane PD, we found that while some of our results confirm the quasi-equilibrium model, other results are in line with the nonequilibrium model. © National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163567
Appears in Collections: 气候变化与战略
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作者单位: Amdursky, N., Schulich Faculty of Chemistry, Technion – Israel Institute of Technology, Haifa, 3200003, Israel; Lin, Y., Department of Materials, Imperial College London, London, SW7 2AZ, United Kingdom; Aho, N., Department of Chemistry, NanoScience Center, University of Jyväskylä, Jyväskylä, FIN-40014, Finland; Groenhof, G., Department of Chemistry, NanoScience Center, University of Jyväskylä, Jyväskylä, FIN-40014, Finland
Recommended Citation:
Amdursky N.,Lin Y.,Aho N.,et al. Exploring fast proton transfer events associated with lateral proton diffusion on the surface of membranes[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(7)