globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1814160116
论文题名:
Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes
作者: Kimata Y.; Kato T.; Higaki T.; Kurihara D.; Yamada T.; Segami S.; Morita M.T.; Maeshima M.; Hasezawa S.; Higashiyama T.; Tasaka M.; Ueda M.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:6
起始页码: 2338
结束页码: 2343
语种: 英语
英文关键词: Apical-basal axis ; Arabidopsis thaliana ; Live-cell imaging ; Vacuole ; Zygote
Scopus关键词: actin filament ; Arabidopsis ; Article ; cell structure ; cell vacuole ; cellular distribution ; controlled study ; embryo ; image analysis ; live cell imaging ; nonhuman ; nuclear localization signal ; ontogeny ; priority journal ; shoot gravitropism2 gene ; transgene ; zygote ; Arabidopsis ; asymmetric cell division ; cell nucleus ; cell polarity ; cell vacuole ; cytology ; fluorescent antibody technique ; genetics ; metabolism ; mutation ; physiology ; zygote ; Arabidopsis protein ; chloroplast protein ; SGR2 protein, Arabidopsis ; Actin Cytoskeleton ; Arabidopsis ; Arabidopsis Proteins ; Asymmetric Cell Division ; Cell Nucleus ; Cell Polarity ; Chloroplast Proteins ; Fluorescent Antibody Technique ; Mutation ; Vacuoles ; Zygote
英文摘要: In most flowering plants, the asymmetric cell division of the zygote is the initial step in establishing the apical-basal axis of the mature plant. The zygote is polarized, possessing the nucleus at the apical tip and large vacuoles at the basal end. Despite their known polar localization, whether the positioning of the vacuoles and the nucleus is coordinated and what the role of the vacuole is in the asymmetric zygotic division remain elusive. In the present study, we utilized a live-cell imaging system to visualize the dynamics of vacuoles during the entire process of zygote polarization in Arabidopsis. Image analysis revealed that the vacuoles formed tubular strands around the apically migrating nucleus. They gradually accumulated at the basal region and filled the space, resulting in asymmetric distribution in the mature zygote. To assess the role of vacuoles in the zygote, we screened various vacuole mutants and identified that shoot gravitropism2 (sgr2), in which the vacuolar structural change was impaired, failed to form tubular vacuoles and to polarly distribute the vacuole. In sgr2, large vacuoles occupied the apical tip and thus nuclear migration was blocked, resulting in a more symmetric zygotic division. We further observed that tubular vacuole formation and asymmetric vacuolar distribution both depended on the longitudinal array of actin filaments. Overall, our results show that vacuolar dynamics is crucial not only for the polar distribution along actin filaments but also for adequate nuclear positioning, and consequently zygote-division asymmetry. © 2019 National Academy of Sciences. All Rights Reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163569
Appears in Collections:气候变化与战略

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作者单位: Kimata, Y., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan; Kato, T., Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192, Japan; Higaki, T., Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan, International Research Organization for Advanced Science and Technology, Kumamoto University, Chuo-ku, Kumamoto, 860-8555, Japan; Kurihara, D., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan; Yamada, T., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan, Institute of Transformative Bio-Molecules, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan; Segami, S., Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan; Morita, M.T., Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan, National Institute for Basic Biology, Myodaiji, Okazaki, Aichi, 444-8585, Japan; Maeshima, M., Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan; Hasezawa, S., Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan; Higashiyama, T., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan, Institute of Transformative Bio-Molecules, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan; Tasaka, M., Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-0192, Japan; Ueda, M., Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan, Institute of Transformative Bio-Molecules, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan

Recommended Citation:
Kimata Y.,Kato T.,Higaki T.,et al. Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(6)
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