globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1912573116
论文题名:
Ketone body receptor GPR43 regulates lipid metabolism under ketogenic conditions
作者: Miyamoto J.; Ohue-Kitano R.; Mukouyama H.; Nishida A.; Watanabe K.; Igarashi M.; Irie J.; Tsujimoto G.; Satoh-Asahara N.; Itoh H.; Kimura I.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:47
起始页码: 23813
结束页码: 23821
语种: 英语
英文关键词: Fasting ; FFAR2 ; Gut microbiota ; Ketone body ; Low carbohydrate
Scopus关键词: acetoacetic acid ; G protein coupled receptor ; G protein coupled receptor 43 ; ketone body ; lipoprotein lipase ; unclassified drug ; animal cell ; animal tissue ; Article ; body weight loss ; caloric intake ; controlled study ; embryo ; energy metabolism ; enzyme activity ; enzyme blood level ; fasting ; fatty acid blood level ; feeding ; GPR43 gene ; heterologous expression ; intestine flora ; ketogenesis ; ketogenic diet ; lipid analysis ; lipid composition ; lipid metabolism ; low carbohydrate diet ; mouse ; nonhuman ; priority journal ; regulatory mechanism ; starvation
英文摘要: Ketone bodies, including β-hydroxybutyrate and acetoacetate, are important alternative energy sources during energy shortage. β-Hydroxybutyrate also acts as a signaling molecule via specific G protein-coupled receptors (GPCRs); however, the specific associated GPCRs and physiological functions of acetoacetate remain unknown. Here we identified acetoacetate as an endogenous agonist for short-chain fatty acid (SCFA) receptor GPR43 by ligand screening in a heterologous expression system. Under ketogenic conditions, such as starvation and low-carbohydrate diets, plasma acetoacetate levels increased markedly, whereas plasma and cecal SCFA levels decreased dramatically, along with an altered gut microbiota composition. In addition, Gpr43-deficient mice showed reduced weight loss and suppressed plasma lipoprotein lipase activity during fasting and eucaloric ketogenic diet feeding. Moreover, Gpr43-deficient mice exhibited minimal weight decrease after intermittent fasting. These observations provide insight into the role of ketone bodies in energy metabolism under shifts in nutrition and may contribute to the development of preventive medicine via diet and foods. © 2019 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163602
Appears in Collections:气候变化与战略

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作者单位: Miyamoto, J., Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan, Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, Tokyo 100-0004, Japan; Ohue-Kitano, R., Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan, Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, Tokyo 100-0004, Japan, Department of Endocrinology, Metabolism, and Hypertension, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, Kyoto, 612-8555, Japan; Mukouyama, H., Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan; Nishida, A., Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan; Watanabe, K., Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan; Igarashi, M., Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan; Irie, J., Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, Tokyo 100-0004, Japan, Department of Endocrinology, Metabolism, and Nephrology, School of Medicine, Keio University, Shinjuku-ku, Tokyo 160-8582, Japan; Tsujimoto, G., Department of Genomic Drug Discovery Science, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, 606-8501, Japan; Satoh-Asahara, N., Department of Endocrinology, Metabolism, and Hypertension, Clinical Research Institute, National Hospital Organization Kyoto Medical Center, Kyoto, 612-8555, Japan; Itoh, H., Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, Tokyo 100-0004, Japan, Department of Endocrinology, Metabolism, and Nephrology, School of Medicine, Keio University, Shinjuku-ku, Tokyo 160-8582, Japan; Kimura, I., Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan, Japan Agency for Medical Research and Development-Core Research for Evolutionary Medical Science and Technology (AMED-CREST), Japan Agency for Medical Research and Development, Chiyoda-ku, Tokyo 100-0004, Japan

Recommended Citation:
Miyamoto J.,Ohue-Kitano R.,Mukouyama H.,et al. Ketone body receptor GPR43 regulates lipid metabolism under ketogenic conditions[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(47)
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