globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1900401116
论文题名:
Blastocyst activation engenders transcriptome reprogram affecting X-chromosome reactivation and inflammatory trigger of implantation
作者: He B.; Zhang H.; Wang J.; Liu M.; Sun Y.; Guo C.; Lu J.; Wang H.; Kong S.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:33
起始页码: 16621
结束页码: 16630
语种: 英语
英文关键词: Blastocyst diapause and reactivation ; Embryo implantation ; Proinflammatory body ; Transcriptome ; X-chromosome reactivation
Scopus关键词: calgranulin B ; transcriptome ; tumor necrosis factor ; calgranulin B ; transcriptome ; tumor necrosis factor ; animal cell ; animal experiment ; Article ; biosynthesis ; blastocyst ; cell activation ; cell differentiation ; cell proliferation ; cell proliferation assay ; controlled study ; diapause ; dormancy ; embryo development ; endometrium ; extracellular matrix ; female ; genetic transcription ; metabolism ; mouse ; nonhuman ; nuclear reprogramming ; priority journal ; protein expression ; real time polymerase chain reaction ; RNA sequence ; signal transduction ; tight junction ; transcriptomics ; upregulation ; X chromosome linkage ; animal ; blastocyst ; cytology ; ectoderm ; gene expression regulation ; genetics ; inflammation ; nidation ; pathology ; time factor ; ultrastructure ; X chromosome ; Animals ; Blastocyst ; Calgranulin B ; Cell Proliferation ; Ectoderm ; Embryo Implantation ; Endometrium ; Female ; Gene Expression Regulation, Developmental ; Inflammation ; Mice ; Time Factors ; Transcriptome ; Tumor Necrosis Factor-alpha ; Up-Regulation ; X Chromosome
英文摘要: Implantation of the blastocyst into the uterus is the gateway for further embryonic development in mammals. Programming of blastocyst to an implantation-competent state known as blastocyst activation is the determining factor for implantation into the receptive uterus. However, it remains largely unclear how the blastocyst is globally programmed for implantation. Employing a delayed implantation mouse model, we show here that the blastocyst undergoes extensive programming essential for implantation. By analyzing the transcriptional profile of blastocysts with different implantation competency, we reveal the dynamic change in the biosynthesis, metabolism, and proliferation during blastocyst reactivation from diapause. We also demonstrate that reactivation of the X chromosome, one of the most important events during periimplantation of female embryonic development, is not completed even in blastocysts under conditions of dormancy, despite long term suspension in the uterus. Moreover, the mural trophectoderm (TE), but not the polar TE, differentiates to be more invasive through the weakened cell-cell tight junctions and extracellular matrices (ECMs). By analyzing the differentially expressed profile of secretory proteins, we further demonstrate that the blastocyst functions as a proinflammatory body to secrete proinflammatory signals, such as TNFα and S100A9, thereby triggering embryouterine attachment reaction during implantation. Collectively, our data systematically and comprehensively disclose the programming of blastocyst reactivation from diapause for implantation and uncover previously undefined roles of blastocyst during implantation. © 2019 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163603
Appears in Collections:气候变化与战略

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作者单位: He, B., Reproductive Medical Center, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China, State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China; Zhang, H., State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China, Basic Forestry and Proteomics Research Center, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China; Wang, J., Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Liu, M., Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Sun, Y., Reproductive Medical Center, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Guo, C., Reproductive Medical Center, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Lu, J., Reproductive Medical Center, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Wang, H., Reproductive Medical Center, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China; Kong, S., Reproductive Medical Center, First Affiliated Hospital of Xiamen University, Xiamen, Fujian, 361003, China, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, 361102, China

Recommended Citation:
He B.,Zhang H.,Wang J.,et al. Blastocyst activation engenders transcriptome reprogram affecting X-chromosome reactivation and inflammatory trigger of implantation[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(33)
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