globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1902922116
论文题名:
Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport
作者: Ferrari M.L.; Malardé V.; Grassart A.; Salavessa L.; Nigro G.; Descorps-Declere S.; Rohde J.R.; Schnupf P.; Masson V.; Arras G.; Loew D.; Sansonetti P.J.; Sauvonnet N.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:27
起始页码: 13582
结束页码: 13591
语种: 英语
英文关键词: Bacteria ; Endocytosis ; Pathogen ; Polarity ; Secretion
Scopus关键词: bacterial protein ; protein IpaJ ; protein VirA ; unclassified drug ; animal experiment ; animal model ; animal tissue ; Article ; bacterial growth ; bacterial strain ; bioinformatics ; controlled study ; endocytosis ; flow cytometry ; fluorescence microscopy ; Golgi complex ; human ; human cell ; immunomodulation ; in vitro study ; in vivo study ; intestine epithelium ; intracellular transport ; liquid chromatography-mass spectrometry ; nonhuman ; pathogenesis ; phenotype ; plasmid ; priority journal ; protein secretion ; protein targeting ; protein transport ; Shigella flexneri ; shigellosis ; species invasion ; total internal reflection fluorescence microscopy
英文摘要: Intracellular trafficking pathways in eukaryotic cells are essential to maintain organelle identity and structure, and to regulate cell communication with its environment. Shigella flexneri invades and subverts the human colonic epithelium by the injection of virulence factors through a type 3 secretion system (T3SS). In this work, we report the multiple effects of two S. flexneri effectors, IpaJ and VirA, which target small GTPases of the Arf and Rab families, consequently inhibiting several intracellular trafficking pathways. IpaJ and VirA induce large-scale impairment of host protein secretion and block the recycling of surface receptors. Moreover, these two effectors decrease clathrin-dependent and -independent endocytosis. Therefore, S. flexneri infection induces a global blockage of host cell intracellular transport, affecting the exchange between cells and their external environment. The combined action of these effectors disorganizes the epithelial cell polarity, disturbs epithelial barrier integrity, promotes multiple invasion events, and enhances the pathogen capacity to penetrate into the colonic tissue in vivo. © 2019 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163617
Appears in Collections:气候变化与战略

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作者单位: Ferrari, M.L., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Malardé, V., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Grassart, A., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Salavessa, L., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France, Université Paris Sud, Paris-Saclay University, Orsay, 91400, France; Nigro, G., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France; Descorps-Declere, S., Institut Pasteur–Hub Bioinformatique et Biostatistique–Centre de Bioinformatique, Biostatistique et Biologie Intégrative, Unité de Services et de Recherche 3756 IP CNRS, Paris, 75015, France; Rohde, J.R., Department of Microbiology and Immunology, Dalhousie University, Halifax, NS B3H 4R2, Canada; Schnupf, P., Institut Necker Enfants Malades, INSERM–CNRS, Laboratory of Host–Microbiota Interaction, Paris, 75015, France; Masson, V., Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris Cedex 05, 75248, France; Arras, G., Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris Cedex 05, 75248, France; Loew, D., Institut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de Masse Protéomique, Paris Cedex 05, 75248, France; Sansonetti, P.J., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France, Chaire de Microbiologie et Maladies Infectieuses, Collège de France, Paris, 75005, France; Sauvonnet, N., Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, 75015, France, INSERM U1202, Paris, 75015, France

Recommended Citation:
Ferrari M.L.,Malardé V.,Grassart A.,et al. Shigella promotes major alteration of gut epithelial physiology and tissue invasion by shutting off host intracellular transport[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(27)
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