globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1905179116
论文题名:
Unexpected host dependency of Antarctic Nanohaloarchaeota
作者: Hamm J.N.; Erdmann S.; Eloe-Fadrosh E.A.; Angeloni A.; Zhong L.; Brownlee C.; Williams T.J.; Barton K.; Carswell S.; Smith M.A.; Brazendale S.; Hancock A.M.; Allen M.A.; Raftery M.J.; Cavicchioli R.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2019
卷: 116, 期:29
起始页码: 14661
结束页码: 14670
语种: 英语
英文关键词: Archaea ; DPANN ; Symbiont
Scopus关键词: Antarctic Nanohaloarchaeota ; Article ; bacterial genome ; bacterial growth ; carboxy terminal sequence ; cell adhesion ; controlled study ; fluorescence activated cell sorting ; fluorescence in situ hybridization ; genetic variation ; genome analysis ; Halorubrum lacusprofundi ; metagenome ; metagenomics ; Nanoarchaeota ; nonhuman ; priority journal ; proteomics ; species invasion ; transmission electron microscopy
英文摘要: In hypersaline environments, Nanohaloarchaeota (Diapherotrites, Parvarchaeota, Aenigmarchaeota, Nanoarchaeota, Nanohaloarchaeota [DPANN] superphylum) are thought to be free-living microorganisms. We report cultivation of 2 strains of Antarctic Nanohaloarchaeota and show that they require the haloarchaeon Halorubrum lacusprofundi for growth. By performing growth using enrichments and fluorescence-activated cell sorting, we demonstrated successful cultivation of Candidatus Nanohaloarchaeum antarcticus, purification of Ca. Nha. antarcticus away from other species, and growth and verification of Ca. Nha. antarcticus with Hrr. lacusprofundi; these findings are analogous to those required for fulfilling Koch’s postulates. We use fluorescent in situ hybridization and transmission electron microscopy to assess cell structures and interactions; metagenomics to characterize enrichment taxa, generate metagenome assembled genomes, and interrogate Antarctic communities; and proteomics to assess metabolic pathways and speculate about the roles of certain proteins. Metagenome analysis indicates the presence of a single species, which is endemic to Antarctic hypersaline systems that support the growth of haloarchaea. The presence of unusually large proteins predicted to function in attachment and invasion of hosts plus the absence of key biosynthetic pathways (e.g., lipids) in metagenome assembled genomes of globally distributed Nanohaloarchaeota indicate that all members of the lineage have evolved as symbionts. Our work expands the range of archaeal symbiotic lifestyles and provides a genetically tractable model system for advancing understanding of the factors controlling microbial symbiotic relationships. © 2019 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163622
Appears in Collections:气候变化与战略

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作者单位: Hamm, J.N., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Erdmann, S., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia, Department of Microbiology, Max Planck Institute for Marine Microbiology, Bremen, 28359, Germany; Eloe-Fadrosh, E.A., Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, United States; Angeloni, A., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Zhong, L., Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, NSW 2052, Australia; Brownlee, C., Biological Resources Imaging Laboratory, University of New South Wales, Sydney, NSW 2052, Australia; Williams, T.J., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Barton, K., Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia; Carswell, S., Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia; Smith, M.A., Kinghorn Centre for Clinical Genomics, Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia, St. Vincent’s Clinical School, University of New South Wales, Sydney, Darlinghurst, NSW 2010, Australia; Brazendale, S., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia, Pegarah, TAS 7256, Australia; Hancock, A.M., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia, University of Tasmania Institute of Marine and Antarctic Studies, Antarctic Gateway Partnership, Antarctic Climate and Ecosystem Research Centre, Battery Point, TAS 7004, Australia; Allen, M.A., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Raftery, M.J., Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney, NSW 2052, Australia; Cavicchioli, R., School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia

Recommended Citation:
Hamm J.N.,Erdmann S.,Eloe-Fadrosh E.A.,et al. Unexpected host dependency of Antarctic Nanohaloarchaeota[J]. Proceedings of the National Academy of Sciences of the United States of America,2019-01-01,116(29)
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