DOI: 10.1073/pnas.1807073115
论文题名: Extensile motor activity drives coherent motions in a model of interphase chromatin
作者: Saintillan D. ; Shelley M.J. ; Zidovska A.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期: 45 起始页码: 11442
结束页码: 11447
语种: 英语
英文关键词: Active matter
; Chromatin
; Nucleoplasm
; Polymer dynamics
Scopus关键词: molecular motor
; adenosine triphosphate
; green fluorescent protein
; histone
; Article
; cell nucleus
; chromatin
; chromatin structure
; chromosomes and chromosomal phenomena
; coherent motion
; conformation
; correlational study
; dipole
; fluid flow
; hydrodynamics
; interphase
; motor activity
; priority journal
; qualitative analysis
; simulation
; structure analysis
; wild type
; chromatin
; gene expression
; genetics
; HeLa cell line
; human
; liquid crystal
; metabolism
; molecular model
; ultrastructure
; Adenosine Triphosphate
; Chromatin
; Gene Expression
; Green Fluorescent Proteins
; HeLa Cells
; Histones
; Humans
; Interphase
; Liquid Crystals
; Models, Molecular
; Molecular Motor Proteins
英文摘要: The 3D spatiotemporal organization of the human genome inside the cell nucleus remains a major open question in cellular biology. In the time between two cell divisions, chromatin-the functional form of DNA in cells-fills the nucleus in its uncon-densed polymeric form. Recent in vivo imaging experiments reveal that the chromatin moves coherently, having displacements with long-ranged correlations on the scale of micrometers and lasting for seconds. To elucidate the mechanism(s) behind these motions, we develop a coarse-grained active polymer model where chromatin is represented as a confined flexible chain acted upon by molecular motors that drive fluid flows by exerting dipolar forces on the system. Numerical simulations of this model account for steric and hydrodynamic interactions as well as internal chain mechanics. These demonstrate that coherent motions emerge in systems involving extensile dipoles and are accompanied by large-scale chain reconfigurations and nematic ordering. Comparisons with experiments show good qualitative agreement and support the hypothesis that self-organizing long-ranged hydrodynamic couplings between chromatin-associated active motor proteins are responsible for the observed coherent dynamics. © 2018 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163643
Appears in Collections: 气候变化与战略
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作者单位: Saintillan, D., Department of Mechanical and Aerospace Engineering, University of California, San Diego, San Diego, CA 92093, United States; Shelley, M.J., Center for Computational Biology, Flatiron Institute, New York, NY 10010, United States, Courant Institute, New York University, New York, NY 10012, United States; Zidovska, A., Center for Soft Matter Research, Department of Physics, New York University, New York, NY 10003, United States
Recommended Citation:
Saintillan D.,Shelley M.J.,Zidovska A.. Extensile motor activity drives coherent motions in a model of interphase chromatin[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(45)