globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1811919115
论文题名:
FtsZ filaments have the opposite kinetic polarity of microtubules
作者: Du S.; Pichoff S.; Kruse K.; Lutkenhaus J.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:42
起始页码: 10768
结束页码: 10773
语种: 英语
英文关键词: Capper ; FtsZ ; Treadmilling ; Tubulin ; Z ring
Scopus关键词: bacterial protein ; cytoskeleton protein ; FtsZ protein, Bacteria ; bacterium ; chemistry ; cytoskeleton ; genetics ; kinetics ; metabolism ; microtubule ; mutation ; polymerization ; protein conformation ; statistical model ; Bacteria ; Bacterial Proteins ; Cytoskeletal Proteins ; Cytoskeleton ; Kinetics ; Microtubules ; Models, Statistical ; Mutation ; Polymerization ; Protein Conformation
英文摘要: FtsZ is the ancestral homolog of tubulin and assembles into the Z ring that organizes the division machinery to drive cell division in most bacteria. In contrast to tubulin that assembles into 13 stranded microtubules that undergo dynamic instability, FtsZ assembles into single-stranded filaments that treadmill to distribute the peptidoglycan synthetic machinery at the septum. Here, using longitudinal interface mutants of FtsZ, we demonstrate that the kinetic polarity of FtsZ filaments is opposite to that of microtubules. A conformational switch accompanying the assembly of FtsZ generates the kinetic polarity of FtsZ filaments, which explains the toxicity of interface mutants that function as a capper and reveals the mechanism of cooperative assembly. This approach can also be employed to determine the kinetic polarity of other filament-forming proteins. © 2018 National Academy of Sciences. All Rights Reserved.
Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163650
Appears in Collections:气候变化与战略

Files in This Item:

There are no files associated with this item.


作者单位: Du, S., Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States; Pichoff, S., Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States; Kruse, K., Department of Biochemistry, University of Geneva, Geneva, 1211, Switzerland, Department of Theoretical Physics, University of Geneva, Geneva, 1211, Switzerland, National Center of Competence in Research Chemical Biology, University of Geneva, Geneva, 1211, Switzerland; Lutkenhaus, J., Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS 66160, United States

Recommended Citation:
Du S.,Pichoff S.,Kruse K.,et al. FtsZ filaments have the opposite kinetic polarity of microtubules[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(42)
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Du S.]'s Articles
[Pichoff S.]'s Articles
[Kruse K.]'s Articles
百度学术
Similar articles in Baidu Scholar
[Du S.]'s Articles
[Pichoff S.]'s Articles
[Kruse K.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Du S.]‘s Articles
[Pichoff S.]‘s Articles
[Kruse K.]‘s Articles
Related Copyright Policies
Null
收藏/分享
所有评论 (0)
暂无评论
 

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.