DOI: 10.1073/pnas.1800828115
论文题名: Roles for ClpXP in regulating the circadian clock in Synechococcus elongatus
作者: Cohen S.E. ; McKnight B.M. ; Golden S.S.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期: 33 起始页码: E7805
结束页码: E7813
语种: 英语
英文关键词: Cell division
; Circadian rhythms
; ClpXP protease
; Cyanobacteria
Scopus关键词: bacterial protein
; chaperone
; protein ClpXP
; proteinase
; unclassified drug
; bacterial protein
; chaperone
; endopeptidase Clp
; Article
; cell cycle regulation
; cell division
; circadian rhythm
; complex formation
; controlled study
; enzyme activity
; enzyme regulation
; nonhuman
; priority journal
; protein depletion
; protein function
; Synechococcus elongatus
; circadian rhythm
; enzymology
; genetics
; metabolism
; physiology
; protein unfolding
; Synechococcus
; Bacterial Proteins
; Circadian Rhythm
; Endopeptidase Clp
; Molecular Chaperones
; Protein Unfolding
; Synechococcus
英文摘要: In cyanobacteria, the KaiABC posttranslational oscillator drives circadian rhythms of gene expression and controls the timing of cell division. The Kai-based oscillator can be reconstituted in vitro, demonstrating that the clock can run without protein synthesis and degradation; however, protein degradation is known to be important for clock function in vivo. Here, we report that strains deficient in the ClpXP1P2 protease have, in addition to known long-period circadian rhythms, an exaggerated ability to synchronize with the external environment (reduced “jetlag”) compared with WT strains. Deletion of the ClpX chaperone, but not the protease subunits ClpP1 or ClpP2, results in cell division defects in a manner that is dependent on the expression of a dusk-peaking factor. We propose that chaperone activities of ClpX are required to coordinate clock control of cell division whereas the protease activities of the ClpXP1P2 complex are required to maintain appropriate periodicity of the clock and its synchronization with the external environment. © 2018 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163677
Appears in Collections: 气候变化与战略
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作者单位: Cohen, S.E., Center for Circadian Biology, Division of Biological Sciences, University of California, San Diego, CA 92093, United States, Department of Biological Sciences, California State University, Los Angeles, CA 90032, United States; McKnight, B.M., Center for Circadian Biology, Division of Biological Sciences, University of California, San Diego, CA 92093, United States; Golden, S.S., Center for Circadian Biology, Division of Biological Sciences, University of California, San Diego, CA 92093, United States
Recommended Citation:
Cohen S.E.,McKnight B.M.,Golden S.S.. Roles for ClpXP in regulating the circadian clock in Synechococcus elongatus[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(33)