DOI: 10.1073/pnas.1804130115
论文题名: MIZ1 regulates ECA1 to generate a slow; long-distance phloem-transmitted Ca2+ signal essential for root water tracking in Arabidopsis
作者: Shkolnik D. ; Nuriel R. ; Bonza M.C. ; Costa A. ; Fromm H.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期: 31 起始页码: 8031
结束页码: 8036
语种: 英语
英文关键词: Arabidopsis
; Calcium
; ECA1
; Hydrotropism
; MIZ1
Scopus关键词: adenosine triphosphatase (calcium)
; MIZ1 protein
; plant protein
; unclassified drug
; Arabidopsis protein
; calcium
; ECA1 protein, Arabidopsis
; mizu-kussei1 protein, Arabidopsis
; vesicular transport adaptor protein
; water
; Arabidopsis
; Article
; calcium signaling
; calcium transport
; cell elongation
; chromatin immunoprecipitation
; controlled study
; enzyme regulation
; genetic manipulation
; nonhuman
; phytochemistry
; plant root
; plant water potential
; priority journal
; protein binding
; yeast cell
; Arabidopsis
; calcium signaling
; cytosol
; metabolism
; phloem
; physiology
; plant root
; Adaptor Proteins, Vesicular Transport
; Arabidopsis
; Arabidopsis Proteins
; Calcium
; Calcium Signaling
; Cytosol
; Phloem
; Plant Roots
; Water
英文摘要: Ever since Darwin postulated that the tip of the root is sensitive to moisture differences and that it “transmits an influence to the upper adjoining part, which bends towards the source of moisture” [Darwin C, Darwin F (1880) The Power of Movement in Plants, pp 572–574], the signal underlying this tropic response has remained elusive. Using the FRET-based Cameleon Ca2+ sensor in planta, we show that a water potential gradient applied across the root tip generates a slow, long-distance asymmetric cytosolic Ca2+ signal in the phloem, which peaks at the elongation zone, where it is dispersed laterally and asymmetrically to peripheral cells, where cell elongation occurs. In addition, the MIZ1 protein, whose biochemical function is unknown but is required for root curvature toward water, is indispensable for generating the slow, long-distance Ca2+ signal. Furthermore, biochemical and genetic manipulations that elevate cytosolic Ca2+ levels, including mutants of the endoplasmic reticulum (ER) Ca2+-ATPase isoform ECA1, enhance root curvature toward water. Finally, coimmunoprecipitation of plant proteins and functional complementation assays in yeast cells revealed that MIZ1 directly binds to ECA1 and inhibits its activity. We suggest that the inhibition of ECA1 by MIZ1 changes the balance between cytosolic Ca2+ influx and efflux and generates the cytosolic Ca2+ signal required for water tracking. © 2018 National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163682
Appears in Collections: 气候变化与战略
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作者单位: Shkolnik, D., School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel; Nuriel, R., School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel; Bonza, M.C., Department of Biosciences, University of Milan, Milan, 20133, Italy; Costa, A., Department of Biosciences, University of Milan, Milan, 20133, Italy, Institute of Biophysics, Consiglio Nazionale delle Ricerche, Milan, 20133, Italy; Fromm, H., School of Plant Sciences and Food Security, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 69978, Israel
Recommended Citation:
Shkolnik D.,Nuriel R.,Bonza M.C.,et al. MIZ1 regulates ECA1 to generate a slow; long-distance phloem-transmitted Ca2+ signal essential for root water tracking in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(31)