globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1704543115
论文题名:
Biomaterial surface energy-driven ligand assembly strongly regulates stem cell mechanosensitivity and fate on very soft substrates
作者: Razafiarison T.; Holenstein C.N.; Stauber T.; Jovic M.; Vertudes E.; Loparic M.; Kawecki M.; Bernard L.; Silvan U.; Snedeker J.G.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:18
起始页码: 4631
结束页码: 4636
语种: 英语
英文关键词: Ligand assembly ; Mechanobiology ; PDMS ; Stem cell ; Surface energy
Scopus关键词: biomaterial ; collagen ; dimeticone ; macrogol ; Rho kinase ; biomaterial ; Article ; cell differentiation ; cell fate ; cell spreading ; controlled study ; enzyme activity ; focal adhesion ; human ; human cell ; hydrophilicity ; hydrophobicity ; mechanical stress ; mesenchymal stem cell ; priority journal ; rigidity ; stem cell culture ; surface property ; Young modulus ; cell adhesion ; cell proliferation ; chemistry ; cytology ; mechanotransduction ; mesenchymal stroma cell ; metabolism ; physiology ; signal transduction ; stem cell ; surface tension ; Biocompatible Materials ; Cell Adhesion ; Cell Differentiation ; Cell Proliferation ; Collagen ; Elastic Modulus ; Humans ; Mechanotransduction, Cellular ; Mesenchymal Stromal Cells ; Signal Transduction ; Stem Cells ; Surface Tension
英文摘要: Although mechanisms of cell–material interaction and cellular mechanotransduction are increasingly understood, the mechanical insensitivity of mesenchymal cells to certain soft amorphous biomaterial substrates has remained largely unexplained. We reveal that surface energy-driven supramolecular ligand assembly can regulate mesenchymal stem cell (MSC) sensing of substrate mechanical compliance and subsequent cell fate. Human MSCs were cultured on collagen-coated hydrophobic polydimethylsiloxane (PDMS) and hydrophilic polyethylene-oxide-PDMS (PEO-PDMS) of a range of stiffnesses. Although cell contractility was similarly diminished on soft substrates of both types, cell spreading and osteogenic differentiation occurred only on soft PDMS and not hydrophilic PEO-PDMS (elastic modulus <1 kPa). Substrate surface energy yields distinct ligand topologies with accordingly distinct profiles of recruited transmembrane cell receptors and related focal adhesion signaling. These differences did not differentially regulate Rho-associated kinase activity, but nonetheless regulated both cell spreading and downstream differentiation. © 2018 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163722
Appears in Collections:气候变化与战略

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作者单位: Razafiarison, T., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Holenstein, C.N., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Stauber, T., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Jovic, M., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Vertudes, E., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Loparic, M., Biozentrum, Swiss Nanoscience Institute, University of Basel, Basel, 4056, Switzerland; Kawecki, M., Laboratory for Nanoscale Materials Science, Empa Dübendorf, Dübendorf, 8600, Switzerland; Bernard, L., Laboratory for Nanoscale Materials Science, Empa Dübendorf, Dübendorf, 8600, Switzerland; Silvan, U., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland; Snedeker, J.G., Department of Orthopedics, Balgrist University Hospital, University of Zurich, Zurich, 8008, Switzerland, Institute for Biomechanics, ETH Zurich, Zurich, 8008, Switzerland

Recommended Citation:
Razafiarison T.,Holenstein C.N.,Stauber T.,et al. Biomaterial surface energy-driven ligand assembly strongly regulates stem cell mechanosensitivity and fate on very soft substrates[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(18)
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