DOI: 10.1073/pnas.1721648115
论文题名: Spatial organization and dynamics of RNase E and ribosomes in Caulobacter crescentus
作者: Bayas C.A. ; Wang J. ; Lee M.K. ; Schrader J.M. ; Shapiro L. ; Moerner W.E.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期: 16 起始页码: E3721
结束页码: E3721
语种: 英语
英文关键词: Microscopy
; Ribosomes
; RNA degradation
; RNA processing
; Single-molecule tracking
; Superresolution
Scopus关键词: ribonuclease
; ribosomal protein L1
; ribosome RNA
; RNA degradosome
; unclassified drug
; bacterial protein
; bacterial RNA
; degradosome
; multienzyme complex
; photoprotein
; polyribonucleotide nucleotidyltransferase
; ribonuclease
; ribonuclease E
; ribosome RNA
; RNA helicase
; yellow fluorescent protein, Bacteria
; Article
; bacterial cell
; Caulobacter
; Caulobacter vibrioides
; cell cycle
; cell membrane
; cell nucleus
; cellular distribution
; chromosome
; controlled study
; cytoplasm
; diffusion
; enzyme activity
; enzyme localization
; enzyme structure
; Escherichia coli
; gene locus
; microscopy
; nonhuman
; priority journal
; ribosome
; RNA degradation
; RNA gene
; RNA processing
; RNA synthesis
; RNA transcription
; RNA translation
; three dimensional imaging
; transcription termination
; bacterial chromosome
; biosynthesis
; Caulobacter vibrioides
; cell fractionation
; cell polarity
; DNA template
; enzymology
; fluorescence microscopy
; gene expression regulation
; genetic transcription
; genetics
; metabolism
; procedures
; ribosome
; single molecule imaging
; ultrastructure
; Bacterial Proteins
; Caulobacter crescentus
; Cell Cycle
; Cell Polarity
; Chromosomes, Bacterial
; Endoribonucleases
; Gene Expression Regulation, Bacterial
; Luminescent Proteins
; Microscopy, Fluorescence
; Multienzyme Complexes
; Polyribonucleotide Nucleotidyltransferase
; Ribosomes
; RNA Helicases
; RNA, Bacterial
; RNA, Ribosomal
; Single Molecule Imaging
; Subcellular Fractions
; Templates, Genetic
; Transcription, Genetic
英文摘要: We report the dynamic spatial organization of Caulobacter crescentus RNase E (RNA degradosome) and ribosomal protein L1 (ribosome) using 3D single-particle tracking and superresolution microscopy. RNase E formed clusters along the central axis of the cell, while weak clusters of ribosomal protein L1 were deployed throughout the cytoplasm. These results contrast with RNase E and ribosome distribution in Escherichia coli, where RNase E colocalizes with the cytoplasmic membrane and ribosomes accumulate in polar nucleoid-free zones. For both RNase E and ribosomes in Caulobacter, we observed a decrease in confinement and clustering upon transcription inhibition and subsequent depletion of nascent RNA, suggesting that RNA substrate availability for processing, degradation, and translation facilitates confinement and clustering. Importantly, RNase E cluster positions correlated with the subcellular location of chromosomal loci of two highly transcribed rRNA genes, suggesting that RNase E’s function in rRNA processing occurs at the site of rRNA synthesis. Thus, components of the RNA degradosome and ribosome assembly are spatiotemporally organized in Caulobacter, with chromosomal readout serving as the template for this organization. © 2018 National Academy of Sciences. All Rights Reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163725
Appears in Collections: 气候变化与战略
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作者单位: Bayas, C.A., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Wang, J., Department of Chemistry, Stanford University, Stanford, CA 94305, United States, Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States; Lee, M.K., Department of Chemistry, Stanford University, Stanford, CA 94305, United States; Schrader, J.M., Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States; Shapiro, L., Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, United States, Chan Zuckerberg Biohub, San Francisco, CA 94158, United States; Moerner, W.E., Department of Chemistry, Stanford University, Stanford, CA 94305, United States
Recommended Citation:
Bayas C.A.,Wang J.,Lee M.K.,et al. Spatial organization and dynamics of RNase E and ribosomes in Caulobacter crescentus[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(16)