DOI: 10.1073/pnas.1800054115
论文题名: EF-Tu and EF-G are activated by allosteric effects
作者: Mondal D. ; Warshel A.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期: 13 起始页码: 3386
结束页码: 3391
语种: 英语
英文关键词: Allosteric activation
; EF-G
; EF-Tu
; GTPase
Scopus关键词: elongation factor G
; elongation factor Tu
; guanosine triphosphatase
; phosphate
; elongation factor G
; elongation factor Tu
; guanosine triphosphate
; allosterism
; analytic method
; Article
; calculation
; catalysis
; controlled study
; crystal structure
; empirical valence bond
; energy transfer
; enzyme mechanism
; mutation
; mutational analysis
; one water mechanism
; priority journal
; ribosome
; simulation
; structure analysis
; two water mechanism
; water flooding approach
; allosterism
; binding site
; chemistry
; enzyme active site
; genetics
; metabolism
; molecular model
; Allosteric Regulation
; Binding Sites
; Catalysis
; Catalytic Domain
; Guanosine Triphosphate
; Models, Molecular
; Mutation
; Peptide Elongation Factor G
; Peptide Elongation Factor Tu
英文摘要: Many cellular processes are controlled by GTPases, and gaining quantitative understanding of the activation of such processes has been a major challenge. In particular, it is crucial to obtain reliable free-energy surfaces for the relevant reaction paths both in solution and in GTPases active sites. Here, we revisit the energetics of the activation of EF-G and EF-Tu by the ribosome and explore the nature of the catalysis of the GTPase reaction. The comparison of EF-Tu to EF-G allows us to explore the impact of possible problems with the available structure of EF-Tu. Additionally, mutational effects are used for a careful validation of the emerging conclusions. It is found that the reaction may proceed by both a two-water mechanism and a one-water (GTP as a base) mechanism. However, in both cases, the activation involves a structural allosteric effect, which is likely to be a general-activation mechanism for all GTPases. © 2018 National Academy of Sciences. All Rights Reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163729
Appears in Collections: 气候变化与战略
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作者单位: Mondal, D., Department of Chemistry, University of Southern California, Los Angeles, CA 90089-1062, United States; Warshel, A., Department of Chemistry, University of Southern California, Los Angeles, CA 90089-1062, United States
Recommended Citation:
Mondal D.,Warshel A.. EF-Tu and EF-G are activated by allosteric effects[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(13)