globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1720237115
论文题名:
Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system
作者: Simonet P.; Gaget K.; Balmand S.; Lopes M.R.; Parisot N.; Buhler K.; Duport G.; Vulsteke V.; Febvay G.; Heddi A.; Charles H.; Callaerts P.; Calevro F.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2018
卷: 115, 期:8
起始页码: E1819
结束页码: E1827
语种: 英语
英文关键词: Acyrthosiphon pisum ; Bacteriocytes ; Buchnera aphidicola ; Cell death ; Symbiosis
Scopus关键词: reactive oxygen metabolite ; adult ; animal cell ; animal tissue ; Article ; autophagosome ; bacteriocyte ; Buchnera aphidicola ; cell death ; cell structure ; cell vacuole ; cellular stress response ; controlled study ; cytoplasm ; electron microscopy ; endoplasmic reticulum ; endosymbiont ; genetic procedures ; giant cell ; immunohistochemistry ; lysosome ; nonhuman ; pea aphid ; priority journal ; symbiosis ; animal ; aphid ; Buchnera ; cell death ; microbiology ; physiology ; symbiosis ; Animals ; Aphids ; Buchnera ; Cell Death ; Lysosomes ; Symbiosis
英文摘要: Symbiotic associations play a pivotal role in multicellular life by facilitating acquisition of new traits and expanding the ecological capabilities of organisms. In insects that are obligatorily dependent on intracellular bacterial symbionts, novel host cells (bacteriocytes) or organs (bacteriomes) have evolved for harboring beneficial microbial partners. The processes regulating the cellular life cycle of these endosymbiont-bearing cells, such as the cell-death mechanisms controlling their fate and elimination in response to host physiology, are fundamental questions in the biology of symbiosis. Here we report the discovery of a cell-death process involved in the degeneration of bacteriocytes in the hemipteran insect Acyrthosiphon pisum. This process is activated progressively throughout aphid adulthood and exhibits morphological features distinct from known cell-death pathways. By combining electron microscopy, immunohistochemistry, and molecular analyses, we demonstrated that the initial event of bacteriocyte cell death is the cytoplasmic accumulation of nonautophagic vacuoles, followed by a sequence of cellular stress responses including the formation of autophagosomes in intervacuolar spaces, activation of reactive oxygen species, and Buchnera endosymbiont degradation by the lysosomal system. We showed that this multi-step cell-death process originates from the endoplasmic reticulum, an organelle exhibiting a unique reticular network organization spread throughout the entire cytoplasm and surrounding Buchnera aphidicola endosymbionts. Our findings provide insights into the cellular and molecular processes that coordinate eukaryotic host and endosymbiont homeostasis and death in a symbiotic system and shed light on previously unknown aspects of bacteriocyte biological functioning. © 2018 National Academy of Sciences. All Rights Reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163740
Appears in Collections:气候变化与战略

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作者单位: Simonet, P., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Gaget, K., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Balmand, S., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Lopes, M.R., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Parisot, N., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Buhler, K., Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, Katholieke Universiteit (KU) Leuven, University of Leuven, Leuven, B-3000, Belgium; Duport, G., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Vulsteke, V., Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, Katholieke Universiteit (KU) Leuven, University of Leuven, Leuven, B-3000, Belgium; Febvay, G., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Heddi, A., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Charles, H., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France; Callaerts, P., Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, Katholieke Universiteit (KU) Leuven, University of Leuven, Leuven, B-3000, Belgium; Calevro, F., UMR0203, Biologie Fonctionnelle, Insectes et Interactions BF2I, Institut National des Sciences Appliquées (INSA-Lyon), Institut National de la Recherche Agronomique (INRA), University of Lyon, Villeurbanne, F-69621, France

Recommended Citation:
Simonet P.,Gaget K.,Balmand S.,et al. Bacteriocyte cell death in the pea aphid/Buchnera symbiotic system[J]. Proceedings of the National Academy of Sciences of the United States of America,2018-01-01,115(8)
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