bacterial protein
; PhoP protein
; protein PhoQ
; sigma factor RpoS
; unclassified drug
; Escherichia coli protein
; PhoP protein, E coli
; PhoQ protein, E coli
; Article
; bacterial growth
; bacterial membrane
; conformational transition
; controlled study
; Escherichia coli
; hyperosmotic stress
; nonhuman
; osmolarity
; pressure
; priority journal
; Salmonella
; signal transduction
; thickness
; Escherichia coli
; growth, development and aging
; osmotic pressure
; physiology
; Salmonella
; Escherichia coli
; Escherichia coli Proteins
; Osmotic Pressure
; Salmonella
英文摘要:
The PhoQ/PhoP two-component system plays an essential role in the response of enterobacteria to the environment of their mammalian hosts. It is known to sense several stimuli that are potentially associated with the host, including extracellular magnesium limitation, low pH, and the presence of cationic antimicrobial peptides. Here, we show that the PhoQ/PhoP two-component systems of Escherichia coli and Salmonella can also perceive an osmotic upshift, another key stimulus to which bacteria become exposed within the host. In contrast to most previously established stimuli of PhoQ, the detection of osmotic upshift does not require its periplasmic sensor domain. Instead, we show that the activity of PhoQ is affected by the length of the transmembrane (TM) helix as well as by membrane lateral pressure. We therefore propose that osmosensing relies on a conformational change within the TM domain of PhoQ induced by a perturbation in cell membrane thickness and lateral pressure under hyperosmotic conditions. Furthermore, the response mediated by the PhoQ/PhoP two-component system was found to improve bacterial growth recovery under hyperosmotic stress, partly through stabilization of the sigma factor RpoS. Our findings directly link the PhoQ/PhoP two-component system to bacterial osmosensing, suggesting that this system can mediate a concerted response to most of the established host-related cues.
Yuan, J., Max Planck Institute for Terrestrial Microbiology, Marburg, 35043, Germany, LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, 35043, Germany; Jin, F., Max Planck Institute for Terrestrial Microbiology, Marburg, 35043, Germany, LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, 35043, Germany; Glatter, T., Max Planck Institute for Terrestrial Microbiology, Marburg, 35043, Germany; Sourjik, V., Max Planck Institute for Terrestrial Microbiology, Marburg, 35043, Germany, LOEWE Center for Synthetic Microbiology (SYNMIKRO), Marburg, 35043, Germany
Recommended Citation:
Yuan J.,Jin F.,Glatter T.,et al. Osmosensing by the bacterial PhoQ/PhoP two-component system[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(50)