globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1716002114
论文题名:
Catch bond drives stator mechanosensitivity in the bacterial flagellar motor
作者: Nord A.L.; Gachon E.; Perez-Carrasco R.; Nirody J.A.; Barducci A.; Berry R.M.; Pedaci F.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2017
卷: 114, 期:49
起始页码: 12952
结束页码: 12957
语种: 英语
英文关键词: Bacterial flagellar motor ; Catch bond ; Escherichia coli ; Mechanosensitivity ; Molecular motor
Scopus关键词: adsorption ; Article ; bacterial cell wall ; bacterial flagellum ; bacterial kinetics ; force ; mechanical stress ; nonhuman ; priority journal ; stoichiometry ; torque ; biomechanics ; chemistry ; Escherichia coli ; flagellum ; kinetics ; molecular model ; Escherichia coli protein ; molecular motor ; Biomechanical Phenomena ; Escherichia coli ; Escherichia coli Proteins ; Flagella ; Kinetics ; Models, Molecular ; Molecular Motor Proteins
英文摘要: The bacterial flagellar motor (BFM) is the rotary motor that rotates each bacterial flagellum, powering the swimming and swarming of many motile bacteria. The torque is provided by stator units, ion motive force-powered ion channels known to assemble and disassemble dynamically in the BFM. This turnover is mechanosensitive, with the number of engaged units dependent on the viscous load experienced by the motor through the flagellum. However, the molecular mechanism driving BFM mechanosensitivity is unknown. Here, we directly measure the kinetics of arrival and departure of the stator units in individual motors via analysis of high-resolution recordings of motor speed, while dynamically varying the load on the motor via external magnetic torque. The kinetic rates obtained, robust with respect to the details of the applied adsorption model, indicate that the lifetime of an assembled stator unit increases when a higher force is applied to its anchoring point in the cell wall. This provides strong evidence that a catch bond (a bond strengthened instead of weakened by force) drives mechanosensitivity of the flagellar motor complex. These results add the BFM to a short, but growing, list of systems demonstrating catch bonds, suggesting that this “molecular strategy” is a widespread mechanism to sense and respond to mechanical stress. We propose that force-enhanced stator adhesion allows the cell to adapt to a heterogeneous environmental viscosity and may ultimately play a role in surface-sensing during swarming and biofilm formation. © 2017, National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163769
Appears in Collections:气候变化与战略

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作者单位: Nord, A.L., Centre de Biochimie Structurale (CBS), INSERM, CNRS, Université de Montpellier, Montpellier, 34090, France; Gachon, E., Centre de Biochimie Structurale (CBS), INSERM, CNRS, Université de Montpellier, Montpellier, 34090, France; Perez-Carrasco, R., Department of Mathematics, University College London, London, WC1E 6BT, United Kingdom; Nirody, J.A., Biophysics Graduate Group, University of California, Berkeley, CA 94720, United States; Barducci, A., Centre de Biochimie Structurale (CBS), INSERM, CNRS, Université de Montpellier, Montpellier, 34090, France; Berry, R.M., Department of Physics, Clarendon Laboratory, University of Oxford, Oxford, OX1 2JD, United Kingdom; Pedaci, F., Centre de Biochimie Structurale (CBS), INSERM, CNRS, Université de Montpellier, Montpellier, 34090, France

Recommended Citation:
Nord A.L.,Gachon E.,Perez-Carrasco R.,et al. Catch bond drives stator mechanosensitivity in the bacterial flagellar motor[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(49)
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