DOI: 10.1073/pnas.1706166114
论文题名: Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4
作者: Zhang X. ; Van Heusden G.P.H. ; Hooykaas P.J.J.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2017
卷: 114, 期: 38 起始页码: 10238
结束页码: 10243
语种: 英语
英文关键词: Agrobacterium
; Kinetochore
; Mitosis
; Spt4
; Virulence
Scopus关键词: bacterial protein
; spt4 protein
; unclassified drug
; vird5 protein
; Arabidopsis protein
; bacterial protein
; SPT4 protein, Arabidopsis
; transcription elongation factor
; Agrobacterium tumefaciens
; Article
; bacterial virulence
; cell nucleus
; chromosomal instability
; chromosome segregation
; growth inhibition
; host cell
; kinetochore
; minichromosome
; mitosis
; nonhuman
; priority journal
; protein binding
; protein protein interaction
; Agrobacterium tumefaciens
; amino acid sequence
; Arabidopsis
; kinetochore
; metabolism
; pathogenicity
; physiology
; Agrobacterium tumefaciens
; Amino Acid Sequence
; Arabidopsis
; Arabidopsis Proteins
; Bacterial Proteins
; Chromosome Segregation
; Kinetochores
; Transcriptional Elongation Factors
英文摘要: The bacterium Agrobacterium tumefaciens causes crown gall tumor formation in plants. During infection the bacteria translocate an oncogenic piece of DNA (transferred DNA, T-DNA) into plant cells at the infection site. A number of virulence proteins are cotransported into host cells concomitantly with the T-DNA to effectuate transformation. Using yeast as a model host, we find that one of these proteins, VirD5, localizes to the centromeres/kinetochores in the nucleus of the host cells by its interaction with the conserved protein Spt4. VirD5 promotes chromosomal instability as seen by the high-frequency loss of a minichromosome in yeast. By using both yeast and plant cells with a chromosome that was specifically marked by a lacO repeat, chromosome segregation errors and the appearance of aneuploid cells due to the presence of VirD5 could be visualized in vivo. Thus, VirD5 is a prokaryotic virulence protein that interferes with mitosis. © 2017, National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163794
Appears in Collections: 气候变化与战略
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作者单位: Zhang, X., Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333BE, Netherlands; Van Heusden, G.P.H., Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333BE, Netherlands; Hooykaas, P.J.J., Department of Molecular and Developmental Genetics, Institute of Biology, Leiden University, Leiden, 2333BE, Netherlands
Recommended Citation:
Zhang X.,Van Heusden G.P.H.,Hooykaas P.J.J.. Virulence protein VirD5 of Agrobacterium tumefaciens binds to kinetochores in host cells via an interaction with Spt4[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(38)