DOI: 10.1073/pnas.1705437114
论文题名: A trans-acting leader RNA from a Salmonella virulence gene
作者: Choi E. ; Han Y. ; Cho Y.-J. ; Nam D. ; Lee E.-J.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2017
卷: 114, 期: 38 起始页码: 10232
结束页码: 10237
语种: 英语
英文关键词: Flagellin
; mgtC
; Trans-acting leader RNA
Scopus关键词: bacterial RNA
; flagellin
; messenger RNA
; ribonuclease E
; bacterial protein
; cation transport protein
; flagellin
; FljB protein, Salmonella
; host factor 1
; magnesium
; MgtC protein, Salmonella typhimurium
; ribonuclease
; ribonuclease E
; transactivator protein
; amino acid sequence
; animal cell
; animal experiment
; Article
; bacterial gene
; bacterial survival
; bacterial virulence
; base pairing
; binding site
; cell survival
; comparative study
; controlled study
; DNA probe
; down regulation
; immunofluorescence test
; mouse
; nonhuman
; Northern blotting
; operon
; phenotype
; priority journal
; promoter region
; protein synthesis
; quantitative analysis
; real time polymerase chain reaction
; RNA cleavage
; RNA degradation
; RNA structure
; Salmonella enterica serovar Typhimurium
; sequence analysis
; start codon
; surface property
; 5' untranslated region
; amino acid substitution
; gene expression regulation
; genetics
; macrophage
; metabolism
; microbiology
; pathogenicity
; Salmonella enterica serovar Typhimurium
; 5' Untranslated Regions
; Amino Acid Substitution
; Bacterial Proteins
; Cation Transport Proteins
; Endoribonucleases
; Flagellin
; Gene Expression Regulation, Bacterial
; Host Factor 1 Protein
; Macrophages
; Magnesium
; Operon
; Salmonella typhimurium
; Trans-Activators
英文摘要: Bacteria use flagella to move toward nutrients, find its host, or retract from toxic substances. Because bacterial flagellum is one of the ligands that activate the host innate immune system, its synthesis should be tightly regulated during host infection, which is largely unknown. Here, we report that a bacterial leader mRNA from the mgtCBR virulence operon in the intracellular pathogen Salmonella enterica serovar Typhimurium binds to the fljB coding region of mRNAs in the fljBA operon encoding the FljB phase 2 flagellin, a main component of bacterial flagella and the FljA repressor for the FliC phase 1 flagellin, and degrades fljBA mRNAs in an RNase E-dependent fashion during infection. A nucleotide substitution of the fljB flagellin gene that prevents the mgtC leader RNA-mediated down-regulation increases the fljB-encoded flagellin synthesis, leading to a hypermotile phenotype inside macrophages. Moreover, the fljB nucleotide substitution renders Salmonella hypervirulent, indicating that FljB-based motility must be compromised in the phagosomal compartment where Salmonella resides. This suggests that this pathogen promotes pathogenicity by producing a virulence protein and limits locomotion by a trans-acting leader RNA from the same virulence gene during infection. © 2017, National Academy of Sciences. All rights reserved. Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163795
Appears in Collections: 气候变化与战略
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作者单位: Choi, E., Department of Genetic Engineering, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 17104, South Korea; Han, Y., Department of Genetic Engineering, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 17104, South Korea; Cho, Y.-J., Unit of Antarctic K-Route Expedition, Korea Polar Research Institute, Incheon, 21990, South Korea; Nam, D., Division of Microbiology, Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Suwon, 16419, South Korea; Lee, E.-J., Department of Genetic Engineering, Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 17104, South Korea
Recommended Citation:
Choi E.,Han Y.,Cho Y.-J.,et al. A trans-acting leader RNA from a Salmonella virulence gene[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(38)