constitutively photomorphogenic 1 protein
; plant protein
; unclassified drug
; Arabidopsis protein
; COP1 protein, Arabidopsis
; PINOID protein, Arabidopsis
; protein binding
; protein serine threonine kinase
; ubiquitin protein ligase
; adult
; Arabidopsis
; Article
; enzyme activity
; Escherichia coli
; gene interaction
; hypocotyl
; in vitro study
; nonhuman
; phenotype
; photomorphogenesis
; plant development
; priority journal
; protein degradation
; protein expression
; protein phosphorylation
; protein processing
; protein protein interaction
; regulatory mechanism
; start codon
; stop codon
; Arabidopsis
; gene expression regulation
; light
; metabolism
; phosphorylation
; physiology
; ubiquitination
; Arabidopsis
; Arabidopsis Proteins
; Gene Expression Regulation, Plant
; Light
; Phosphorylation
; Protein Binding
; Protein Processing, Post-Translational
; Protein-Serine-Threonine Kinases
; Ubiquitin-Protein Ligases
; Ubiquitination
英文摘要:
CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) plays crucial roles in various cellular processes via its E3 ubiquitin ligase activity in organisms, ranging from fungi to humans. As a key component in regulating various biological events, COP1 itself is precisely controlled at multiple layers. Here, we report a negative regulator of COP1, PINOID (PID), which positively mediates photomorphogenic development. Specifically, PID genetically and physically interacts with COP1 and directly phosphorylates COP1 at Ser20. As a result, this posttranslational modification serves to repress COP1 activity and promote photomorphogenesis. Our findings signify a key regulatory mechanism for precisely maintaining COP1 activity, thereby ensuring appropriate development in plants.
Lin, F., Department of Biology, Southern University of Science and Technology, Shenzhen, 518055, China, State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences, School of Life Sciences, Peking University, Beijing, 100871, China; Xu, D., Department of Biology, Southern University of Science and Technology, Shenzhen, 518055, China; Jiang, Y., Department of Biology, Southern University of Science and Technology, Shenzhen, 518055, China, State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences, School of Life Sciences, Peking University, Beijing, 100871, China; Chen, H., State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences, School of Life Sciences, Peking University, Beijing, 100871, China; Fan, L., State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences, School of Life Sciences, Peking University, Beijing, 100871, China; Holm, M., Department of Biological and Environmental Sciences, Gothenburg University, Gothenburg, SE-405 30, Sweden; Deng, X.W., Department of Biology, Southern University of Science and Technology, Shenzhen, 518055, China, State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agriculture Sciences, School of Life Sciences, Peking University, Beijing, 100871, China
Recommended Citation:
Lin F.,Xu D.,Jiang Y.,et al. Phosphorylation and negative regulation of CONSTITUTIVELY PHOTOMORPHOGENIC 1 by PINOID in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(25)