globalchange  > 气候变化与战略
DOI: 10.1073/pnas.1700766114
论文题名:
Spemann organizer transcriptome induction by early beta-catenin; Wnt; Nodal; and Siamois signals in Xenopus laevis
作者: Ding Y.; Ploper D.; Sosa E.A.; Colozza G.; Moriyama Y.; Benitez M.D.J.; Zhang K.; Merkurjev D.; De Robertis E.M.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2017
卷: 114, 期:15
起始页码: E3081
结束页码: E3090
语种: 英语
英文关键词: Cerberus ; Embryonic induction ; Embryonic patterning ; Spemann organizer ; Wnt signaling
Scopus关键词: beta catenin ; dickkopf 1 protein ; lithium chloride ; messenger RNA ; morpholino oligonucleotide ; RNA ; transcriptome ; Wnt8a protein ; beta catenin ; homeodomain protein ; protein Nodal ; siamois protein, Xenopus ; transcriptome ; Wnt protein ; Xenopus protein ; Article ; blastula ; embryo ; gastrula ; genome ; morphogenesis ; nonhuman ; priority journal ; signal transduction ; Spemann organizer ; transcription initiation ; ventral region ; Wnt signaling ; Xenopus laevis ; amino acid sequence ; animal ; gene expression regulation ; genetics ; growth, development and aging ; metabolism ; physiology ; sequence homology ; Spemann organizer ; Xenopus laevis ; Amino Acid Sequence ; Animals ; beta Catenin ; Gene Expression Regulation, Developmental ; Homeodomain Proteins ; Nodal Protein ; Organizers, Embryonic ; Sequence Homology ; Transcriptome ; Wnt Proteins ; Xenopus laevis ; Xenopus Proteins
英文摘要: The earliest event in Xenopus development is the dorsal accumulation of nuclear β-catenin under the influence of cytoplasmic determinants displaced by fertilization. In this study, a genome-wide approach was used to examine transcription of the 43,673 genes annotated in the Xenopus laevis genome under a variety of conditions that inhibit or promote formation of the Spemann organizer signaling center. Loss of function of β-catenin with antisense morpholinos reproducibly reduced the expression of 247 mRNAs at gastrula stage. Interestingly, only 123 β-catenin targets were enriched on the dorsal side and defined an early dorsal β-catenin gene signature. These genes included several previously unrecognized Spemann organizer components. Surprisingly, only 3 of these 123 genes overlapped with the late Wnt signature recently defined by two other groups using inhibition by Dkk1 mRNA or Wnt8 morpholinos, which indicates that the effects of β-catenin/ Wnt signaling in early development are exquisitely regulated by stage-dependent mechanisms. We analyzed transcriptome responses to a number of treatments in a total of 46 RNA-seq libraries. These treatments included, in addition to β-catenin depletion, regenerating dorsal and ventral half-embryos, lithium chloride treatment, and the overexpression of Wnt8, Siamois, and Cerberus mRNAs. Only some of the early dorsal β-catenin signature genes were activated at blastula whereas others required the induction of endomesoderm, as indicated by their inhibition by Cerberus overexpression. These comprehensive data provide a rich resource for analyzing how the dorsal and ventral regions of the embryo communicate with each other in a self-organizing vertebrate model embryo. © 2017, National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/163854
Appears in Collections:气候变化与战略

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作者单位: Ding, Y., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Ploper, D., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Sosa, E.A., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Colozza, G., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Moriyama, Y., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Benitez, M.D.J., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Zhang, K., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States; Merkurjev, D., Department of Medicine, University of California, Los Angeles, CA 90095-1662, United States, Department of Microbiology, University of California, Los Angeles, CA 90095-1662, United States, Department of Human Genetics, University of California, Los Angeles, CA 90095-1662, United States; De Robertis, E.M., Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095-1662, United States, Department of Biological Chemistry, University of California, Los Angeles, CA 90095-1662, United States

Recommended Citation:
Ding Y.,Ploper D.,Sosa E.A.,et al. Spemann organizer transcriptome induction by early beta-catenin; Wnt; Nodal; and Siamois signals in Xenopus laevis[J]. Proceedings of the National Academy of Sciences of the United States of America,2017-01-01,114(15)
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