globalchange  > 气候变化与战略
DOI: 10.1073/pnas.2011299117
论文题名:
Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs
作者: Versantvoort W.; Pol A.; Jetten M.S.M.; van Niftrik L.; Reimann J.; Kartal B.; Op den Camp H.J.M.
刊名: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 0027-8424
出版年: 2020
卷: 117, 期:39
起始页码: 24459
结束页码: 24463
语种: 英语
英文关键词: Ammonia oxidation ; Hydroxylamine oxidoreductase ; Methanotrophy ; Multiheme cytochrome ; Nitric oxide
Scopus关键词: ammonia ; hydroxylamine ; hydroxylamine oxidase ; lanthanide ; methane ; methanol dehydrogenase ; nitric oxide ; aerobic ammonia oxidizer ; aerobic metabolism ; anaerobic ammonium oxidation ; Article ; catalysis ; chromatophore ; concentration (parameter) ; ecosystem ; environment ; kinetics ; landfill ; methanotrophic bacterium ; Methylacidiphilum fumariolicum ; nonhuman ; priority journal ; purification ; rice ; thermophilic bacterium ; volcano
英文摘要: Aerobic and nitrite-dependent methanotrophs make a living from oxidizing methane via methanol to carbon dioxide. In addition, these microorganisms cometabolize ammonia due to its structural similarities to methane. The first step in both of these processes is catalyzed by methane monooxygenase, which converts methane or ammonia into methanol or hydroxylamine, respectively. Methanotrophs use methanol for energy conservation, whereas toxic hydroxylamine is a potent inhibitor that needs to be rapidly removed. It is suggested that many methanotrophs encode a hydroxylamine oxidoreductase (mHAO) in their genome to remove hydroxylamine, although biochemical evidence for this is lacking. HAOs also play a crucial role in the metabolism of aerobic and anaerobic ammonia oxidizers by converting hydroxylamine to nitric oxide (NO). Here, we purified an HAO from the thermophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum SolV and characterized its kinetic properties. This mHAO possesses the characteristic P460 chromophore and is active up to at least 80 °C. It catalyzes the rapid oxidation of hydroxylamine to NO. In methanotrophs, mHAO efficiently removes hydroxylamine, which severely inhibits calcium-dependent, and as we show here, lanthanide-dependent methanol dehydrogenases, which are more prevalent in the environment. Our results indicate that mHAO allows methanotrophs to thrive under high ammonia concentrations in natural and engineered ecosystems, such as those observed in rice paddy fields, landfills, or volcanic mud pots, by preventing the accumulation of inhibitory hydroxylamine. Under oxic conditions, methanotrophs mainly oxidize ammonia to nitrite, whereas in hypoxic and anoxic environments reduction of both ammonia-derived nitrite and NO could lead to nitrous oxide (N2O) production. © 2020 National Academy of Sciences. All rights reserved.
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/164022
Appears in Collections:气候变化与战略

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作者单位: Versantvoort, W., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Pol, A., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Jetten, M.S.M., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; van Niftrik, L., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Reimann, J., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands; Kartal, B., Microbial Physiology Group, Max Planck Institute for Marine Microbiology, Bremen, 28359, Germany; Op den Camp, H.J.M., Department of Microbiology, Institute for Water and Wetland Research, Faculty of Science, Radboud University, Nijmegen, 6525 AJ, Netherlands

Recommended Citation:
Versantvoort W.,Pol A.,Jetten M.S.M.,et al. Multiheme hydroxylamine oxidoreductases produce NO during ammonia oxidation in methanotrophs[J]. Proceedings of the National Academy of Sciences of the United States of America,2020-01-01,117(39)
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