globalchange  > 气候变化与战略
DOI: 10.1016/j.scib.2020.08.011
论文题名:
Dynamic global analysis of transcription reveals the role of miRNAs in synergistic stabilization of gene expression
作者: Ma F.; Lu G.-A.; Chen Q.; Ruan Y.; Li X.; Lu X.; Li C.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2020
卷: 65, 期:24
起始页码: 2130
结束页码: 2140
语种: 英语
中文关键词: miRNAs ; Perturbation ; Stability ; Synergistic effects ; Transcriptome
英文关键词: Dynamics ; Plants (botany) ; Recovery ; Transcription ; Exogenous perturbation ; Experimental evidence ; Expression dynamics ; Genetic backgrounds ; Inhibition of transcriptions ; miRNA expressions ; Transcriptional regulatory networks ; Transient perturbation ; RNA
英文摘要: Buffering exogenous perturbation is crucial to maintain transcriptional homeostasis during development. While miRNAs have been speculated to play a role in stability maintenance, previous studies seeking to check this conjecture focused on measurements of transcript levels at steady state or involved individual miRNA targets. We measured whole-genome expression dynamics by introducing a transient perturbation and establishing a perturbation and recovery system in Drosophila larvae. We inhibited all transcription and assayed transcriptomes at several time points during recovery from inhibition. We performed these experiments in the wild type and miRNA-deficient genetic backgrounds. Consistent with theories about miRNAs’ function in stabilizing the transcriptome, we find that attenuating miRNA expression leads to weak impairment in degradation of targets but strong destabilization of target genes when transcription is re-activated. We further fitted a model that captures the essential aspects of transcription dynamics in our experiments and found that the miRNA target transcripts uniformly overshoot the original steady state as they recover from a general inhibition of transcription if global miRNA levels are reduced. Collectively, our results provide experimental evidence for the idea that miRNAs act cumulatively to stabilize the transcriptional regulatory network. We therefore found a promising approach to assess the effect of these molecules on transcription dynamics. © 2020 Science China Press
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170001
Appears in Collections:气候变化与战略

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作者单位: School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China; Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine and Engineering, and Key Laboratory of Big Data-Based Precision Medicine (Ministry of Industry and Information Technology), Beihang University, Beijing 100191, China; State Key Laboratory of Genetic Resources and Evolution & CAS Center for Excellence in Animal Evolution and Genetics, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; Key Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China

Recommended Citation:
Ma F.,Lu G.-A.,Chen Q.,et al. Dynamic global analysis of transcription reveals the role of miRNAs in synergistic stabilization of gene expression[J]. Science Bulletin,2020-01-01,65(24)
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