globalchange  > 气候变化与战略
DOI: 10.1016/j.scib.2020.03.026
论文题名:
Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice
作者: Sun L.; Chen R.; Li L.; Yuan B.; Song K.; Pan N.; Cheng T.-L.; Chang S.; Lin K.; He X.; Wu Q.; Xu F.; Qiu Z.; Wang X.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2020
卷: 65, 期:14
起始页码: 1192
结束页码: 1202
语种: 英语
中文关键词: Autism ; CRISPR/Cas9 ; In vivo calcium imaging ; MECP2 ; Social activity
英文关键词: Brain ; Defects ; Electrophysiology ; Neurons ; Activity patterns ; Calcium imaging ; Genomic segments ; Neural activity ; Neural circuits ; Neural ensembles ; Social interactions ; Therapeutic targets ; Mammals
英文摘要: Duplications of MECP2-containing genomic segments led to severe autistic symptoms in male. Transgenic mice overexpressing the human MECP2 gene exhibit autistic-like behaviors. Neural circuits underlying social defects in MECP2 transgenic (MECP2-TG) mice remain unknown. To observe neural activity of MECP2-TG mice in vivo, we performed calcium imaging by implantation of microendoscope in the hippocampal CA1 regions of MECP2-TG and wild type (WT) mice. We identified neurons whose activities were tightly associated with social interaction, which activity patterns were compromised in MECP2-TG mice. Strikingly, we rescued the social-related neural activity in CA1 and social defects in MECP2-TG mice by deleting the human MECP2 transgene using the CRISPR/Cas9 method during adulthood. Our data points to the neural circuitry responsible for social interactions and provides potential therapeutic targets for autism in adulthood. © 2020 Science China Press
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170039
Appears in Collections:气候变化与战略

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作者单位: State Key Laboratory of Brain and Cognitive Sciences, CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Brain-Intelligence Technology (Shanghai), Institute for Stem Cell and Regeneration, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China; Institute of Neuroscience, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China; The College of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, China; The College of Life Science, Hebei Normal University, Shijiazhuang, 050016, China; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Wuhan, 430071, China; Advanced Innovation Center for Human Brain Protection, Beijing Institute for Brain Disorders, Capital Medical University, Beijing, 100069, China

Recommended Citation:
Sun L.,Chen R.,Li L.,et al. Visualization and correction of social abnormalities-associated neural ensembles in adult MECP2 duplication mice[J]. Science Bulletin,2020-01-01,65(14)
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