DOI: 10.1016/j.scib.2021.06.010
论文题名: Genomewide decoupling of H2AK119ub1 and H3K27me3 in early mouse development
作者: Zhu Y. ; Yu J. ; Rong Y. ; Wu Y.-W. ; Li Y. ; Zhang L. ; Pan Y. ; Fan H.-Y. ; Shen L.
刊名: Science Bulletin
ISSN: 20959273
出版年: 2021
语种: 英语
中文关键词: H2AK119ub1
; H3K27me3
; Non-canonical imprinting
; Polycomb
; Preimplantation development
英文关键词: Alkylation
; Gene encoding
; Chromatin immunoprecipitation
; Complex 1
; Decouplings
; H2ak119ub1
; H3k27me3
; Non-canonical imprinting
; Polycomb
; Polycomb group proteins
; Preimplantation development
; Ultra sensitives
; Mammals
英文摘要: Polycomb group (PcG) proteins are crucial chromatin regulators during development. H2AK119ub1 (H2Aub) and H3K27me3 are catalyzed by Polycomb-repressive complex 1 and 2 (PRC1/2) respectively, and they largely overlap in the genome due to mutual recruitment of the two complexes. However, it is unclear whether PRC1/H2Aub and PRC2/H3K27me3 can also function independently. By developing an ultra-sensitive carrier-DNA-assisted chromatin immunoprecipitation sequencing method termed CATCH-Seq, we generated allelic H2Aub profiles in mouse gametes and early embryos. Our results revealed an unexpected genomewide decoupling of H2Aub and H3K27me3 in mouse preimplantation embryos, where H2Aub but not H3K27me3 was enriched at PcG targets while only H3K27me3 was deposited in the broad distal domains associated with DNA methylation-independent non-canonical imprinting. These observations suggest that H2Aub represses future bivalent genes during early embryogenesis without H3K27me3, but it is not required for the maintenance of non-canonical imprinting, which is mediated by maternal H3K27me3. Thus, our study reveals the distinct depositions and independent functions of H2Aub and H3K27me3 during early mammalian development. © 2021 Science China Press Citation statistics:
资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/170534
Appears in Collections: 气候变化与战略
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作者单位: The MOE Key Laboratory of Biosystems Homeostasis & Protection and Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University, Hangzhou, 310058, China; Department of Orthopedics Surgery, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China; Hangzhou Innovation Center, Zhejiang University, Hangzhou, 311215, China
Recommended Citation:
Zhu Y.,Yu J.,Rong Y.,et al. Genomewide decoupling of H2AK119ub1 and H3K27me3 in early mouse development[J]. Science Bulletin,2021-01-01