globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0098307
论文题名:
Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktivplus in the Lake Constance Area, Germany
作者: Anja Henneberg; Katrin Bender; Ludek Blaha; Sabrina Giebner; Bertram Kuch; Heinz-R. Köhler; Diana Maier; Jörg Oehlmann; Doreen Richter; Marco Scheurer; Ulrike Schulte-Oehlmann; Agnes Sieratowicz; Simone Ziebart; Rita Triebskorn
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-6-5
卷: 9, 期:6
语种: 英语
英文关键词: Sediment ; Trout ; Rivers ; Surface water ; Water pollution ; Embryos ; Reporter gene assay ; Reproductive endocrinology
英文摘要: Many studies about endocrine pollution in the aquatic environment reveal changes in the reproduction system of biota. We analysed endocrine activities in two rivers in Southern Germany using three approaches: (1) chemical analyses, (2) in vitro bioassays, and (3) in vivo investigations in fish and snails. Chemical analyses were based on gas chromatography coupled with mass spectrometry. For in vitro analyses of endocrine potentials in water, sediment, and waste water samples, we used the E-screen assay (human breast cancer cells MCF-7) and reporter gene assays (human cell line HeLa-9903 and MDA-kb2). In addition, we performed reproduction tests with the freshwater mudsnail Potamopyrgus antipodarum to analyse water and sediment samples. We exposed juvenile brown trout (Salmo trutta f. fario) to water downstream of a wastewater outfall (Schussen River) or to water from a reference site (Argen River) to investigate the vitellogenin production. Furthermore, two feral fish species, chub (Leuciscus cephalus) and spirlin (Alburnoides bipunctatus), were caught in both rivers to determine their gonadal maturity and the gonadosomatic index. Chemical analyses provided only little information about endocrine active substances, whereas the in vitro assays revealed endocrine potentials in most of the samples. In addition to endocrine potentials, we also observed toxic potentials (E-screen/reproduction test) in waste water samples, which could interfere with and camouflage endocrine effects. The results of our in vivo tests were mostly in line with the results of the in vitro assays and revealed a consistent reproduction-disrupting (reproduction tests) and an occasional endocrine action (vitellogenin levels) in both investigated rivers, with more pronounced effects for the Schussen river (e.g. a lower gonadosomatic index). We were able to show that biological in vitro assays for endocrine potentials in natural stream water reasonably reflect reproduction and endocrine disruption observed in snails and field-exposed fish, respectively.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0098307&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/17916
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Animal Physiological Ecology, University of Tübingen, Tübingen, Germany;Department Aquatic Ecotoxicology, University of Frankfurt am Main, Frankfurt am Main, Germany;Faculty of Science, RECETOX, Masaryk University, Brno, Czech Republic;Department Aquatic Ecotoxicology, University of Frankfurt am Main, Frankfurt am Main, Germany;Institute for Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Stuttgart, Germany;Animal Physiological Ecology, University of Tübingen, Tübingen, Germany;Animal Physiological Ecology, University of Tübingen, Tübingen, Germany;Department Aquatic Ecotoxicology, University of Frankfurt am Main, Frankfurt am Main, Germany;Water Technology Center Karlsruhe, Karlsruhe, Germany;Water Technology Center Karlsruhe, Karlsruhe, Germany;Department Aquatic Ecotoxicology, University of Frankfurt am Main, Frankfurt am Main, Germany;Department Aquatic Ecotoxicology, University of Frankfurt am Main, Frankfurt am Main, Germany;Department Aquatic Ecotoxicology, University of Frankfurt am Main, Frankfurt am Main, Germany;Animal Physiological Ecology, University of Tübingen, Tübingen, Germany

Recommended Citation:
Anja Henneberg,Katrin Bender,Ludek Blaha,et al. Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktivplus in the Lake Constance Area, Germany[J]. PLOS ONE,2014-01-01,9(6)
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