globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0106644
论文题名:
Exposure to Paper Mill Effluent at a Site in North Central Florida Elicits Molecular-Level Changes in Gene Expression Indicative of Progesterone and Androgen Exposure
作者: Erica K. Brockmeier; B. Sumith Jayasinghe; William E. Pine; Krystan A. Wilkinson; Nancy D. Denslow
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-9-8
卷: 9, 期:9
语种: 英语
英文关键词: Gene expression ; Androgens ; Progesterone ; Water pollution ; Surface water ; Effluent ; Metabolic processes ; Oocytes
英文摘要: Endocrine disrupting compounds (EDCs) are chemicals that negatively impact endocrine system function, with effluent from paper mills one example of this class of chemicals. In Florida, female Eastern mosquitofish (Gambusia holbrooki) have been observed with male secondary sexual characteristics at three paper mill-impacted sites, indicative of EDC exposure, and are still found at one site on the Fenholloway River. The potential impacts that paper mill effluent exposure has on the G. holbrooki endocrine system and the stream ecosystem are unknown. The objective of this study was to use gene expression analysis to determine if exposure to an androgen receptor agonist was occurring and to couple this analysis with in vitro assays to evaluate the presence of androgen and progesterone receptor active chemicals in the Fenholloway River. Focused gene expression analyses of masculinized G. holbrooki from downstream of the Fenholloway River paper mill were indicative of androgen exposure, while genes related to reproduction indicated potential progesterone exposure. Hepatic microarray analysis revealed an increase in the expression of metabolic genes in Fenholloway River fish, with similarities in genes and biological processes compared to G. holbrooki exposed to androgens. Water samples collected downstream of the paper mill and at a reference site indicated that progesterone and androgen receptor active chemicals were present at both sites, which corroborates previous chemical analyses. Results indicate that G. holbrooki downstream of the Fenholloway River paper mill are impacted by a mixture of both androgens and progesterones. This research provides data on the mechanisms of how paper mill effluents in Florida are acting as endocrine disruptors.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0106644&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/18182
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Department of Physiological Sciences, Center for Environmental and Human Toxicology, University of Florida, Gainesville, Florida, United States of America;Department of Physiological Sciences, Center for Environmental and Human Toxicology, University of Florida, Gainesville, Florida, United States of America;Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, Florida, United States of America;Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, Florida, United States of America;Chicago Zoological Society, c/o Mote Marine Laboratory, Sarasota, Florida, United States of America;Department of Physiological Sciences, Center for Environmental and Human Toxicology, University of Florida, Gainesville, Florida, United States of America;Genetics Institute, University of Florida, Gainesville, Florida, United States of America

Recommended Citation:
Erica K. Brockmeier,B. Sumith Jayasinghe,William E. Pine,et al. Exposure to Paper Mill Effluent at a Site in North Central Florida Elicits Molecular-Level Changes in Gene Expression Indicative of Progesterone and Androgen Exposure[J]. PLOS ONE,2014-01-01,9(9)
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