globalchange  > 过去全球变化的重建
DOI: 10.1371/journal.pone.0095333
论文题名:
In Vitro Calcite Crystal Morphology Is Modulated by Otoconial Proteins Otolin-1 and Otoconin-90
作者: K. Trent Moreland; Mina Hong; Wenfu Lu; Christopher W. Rowley; David M. Ornitz; James J. De Yoreo; Ruediger Thalmann
刊名: PLOS ONE
ISSN: 1932-6203
出版年: 2014
发表日期: 2014-4-18
卷: 9, 期:4
语种: 英语
英文关键词: Calcite ; Crystal growth ; Crystals ; Atomic force microscopy ; Mica ; Recombinant proteins ; Collagens ; Biomineralization
英文摘要: Otoconia are formed embryonically and are instrumental in detecting linear acceleration and gravity. Degeneration and fragmentation of otoconia in elderly patients leads to imbalance resulting in higher frequency of falls that are positively correlated with the incidence of bone fractures and death. In this work we investigate the roles otoconial proteins Otolin-1 and Otoconin 90 (OC90) perform in the formation of otoconia. We demonstrate by rotary shadowing and atomic force microscopy (AFM) experiments that Otolin-1 forms homomeric protein complexes and self-assembled networks supporting the hypothesis that Otolin-1 serves as a scaffold protein of otoconia. Our calcium carbonate crystal growth data demonstrate that Otolin-1 and OC90 modulate in vitro calcite crystal morphology but neither protein is sufficient to produce the shape of otoconia. Coadministration of these proteins produces synergistic effects on crystal morphology that contribute to morphology resembling otoconia.
URL: http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0095333&type=printable
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资源类型: 期刊论文
标识符: http://119.78.100.158/handle/2HF3EXSE/18207
Appears in Collections:过去全球变化的重建
影响、适应和脆弱性
科学计划与规划
气候变化与战略
全球变化的国际研究计划
气候减缓与适应
气候变化事实与影响

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作者单位: Department of Otolaryngology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, United States of America;Department of Chemistry, The George Washington University, Washington, D.C., United States of America;Department of Otolaryngology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, United States of America;Department of Otolaryngology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, United States of America;Department of Developmental Biology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, United States of America;The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, California, United States of America;Department of Otolaryngology, Washington University in St. Louis School of Medicine, St. Louis, Missouri, United States of America

Recommended Citation:
K. Trent Moreland,Mina Hong,Wenfu Lu,et al. In Vitro Calcite Crystal Morphology Is Modulated by Otoconial Proteins Otolin-1 and Otoconin-90[J]. PLOS ONE,2014-01-01,9(4)
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