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DOI:	10.1371/journal.pone.0113408
论文题名:	Functionalized Graphene Sheets As Immobilization Matrix for Fenugreek β-Amylase: Enzyme Kinetics and Stability Studies
作者:	Garima Srivastava; Kritika Singh; Mahe Talat; Onkar Nath Srivastava; Arvind M. Kayastha
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-11-20
卷:	9, 期:11
语种:	英语
英文关键词:	Enzyme immobilization ; Experimental design ; Enzyme kinetics ; Graphite ; Enzyme assays ; Enzymes ; Scanning electron microscopy ; Starches
英文摘要:	β-Amylase finds application in food and pharmaceutical industries. Functionalized graphene sheets were customised as a matrix for covalent immobilization of Fenugreek β-amylase using glutaraldehyde as a cross-linker. The factors affecting the process were optimized using Response Surface Methodology based Box-Behnken design of experiment which resulted in 84% immobilization efficiency. Scanning and Transmission Electron Microscopy (SEM, TEM) and Fourier Tansform Infrared (FTIR) spectroscopy were employed for the purpose of characterization of attachment of enzyme on the graphene. The enzyme kinetic studies were carried out for obtaining best catalytic performance and enhanced reusability. Optimum temperature remained unchanged, whereas optimum pH showed shift towards acidic range for immobilized enzyme. Increase in thermal stability of immobilized enzyme and non-toxic nature of functionalized graphene can be exploited for production of maltose in food and pharmaceutical industries.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0113408&type=printable
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资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/18469
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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作者单位:

School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India;School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India;Nanoscience and Nanotechnology Unit, Department of Physics, Banaras Hindu University, Varanasi, India;Nanoscience and Nanotechnology Unit, Department of Physics, Banaras Hindu University, Varanasi, India;School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India

Recommended Citation:

Garima Srivastava,Kritika Singh,Mahe Talat,et al. Functionalized Graphene Sheets As Immobilization Matrix for Fenugreek β-Amylase: Enzyme Kinetics and Stability Studies[J]. PLOS ONE,2014-01-01,9(11)

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