Surface Immuno-Functionalisation for the Capture and Detection of Vibrio Species in the Marine Environment: A New Management Tool for Industrial Facilities
Bacteria from the genus Vibrio are a common and environmentally important group of bacteria within coastal environments and include species pathogenic to aquaculture organisms. Their distribution and abundance are linked to specific environmental parameters, including temperature, salinity and nutrient enrichment. Accurate and efficient detection of Vibrios in environmental samples provides a potential important indicator of overall ecosystem health while also allowing rapid management responses for species pathogenic to humans or species implicated in disease of economically important aquacultured fish and invertebrates. In this study, we developed a surface immuno-functionalisation protocol, based on an avidin-biotin type covalent binding strategy, allowing specific sandwich-type detection of bacteria from the Vibrio genus. The assay was optimized on 12 diverse Vibrio strains, including species that have implications for aquaculture industries, reaching detection limits between 7×103 to 3×104 cells mL−1. Current techniques for the detection of total Vibrios rely on laborious or inefficient analyses resulting in delayed management decisions. This work represents a novel approach for a rapid, accurate, sensitive and robust tool for quantifying Vibrios directly in industrial systems and in the environment, thereby facilitating rapid management responses.
Plant Functional Biology and Climate Change Cluster (C3), University of Technology, Sydney, NSW, Australia;The ithree institute, University of Technology, Sydney, NSW, Australia;Plant Functional Biology and Climate Change Cluster (C3), University of Technology, Sydney, NSW, Australia;Australian Institute for Marine Science, Townsville, QLD, Australia;Port Stephens Fisheries Institute, New South Wales Department of Primary Industries, Taylors Beach, NSW, Australia;Plant Functional Biology and Climate Change Cluster (C3), University of Technology, Sydney, NSW, Australia
Recommended Citation:
Olivier F. Laczka,Maurizio Labbate,Justin R. Seymour,et al. Surface Immuno-Functionalisation for the Capture and Detection of Vibrio Species in the Marine Environment: A New Management Tool for Industrial Facilities[J]. PLOS ONE,2014-01-01,9(10)