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DOI:	10.1371/journal.pone.0104629
论文题名:	Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis
作者:	Brenda A. Álvarez-Sandoval; Linda R. Manzanilla; Rafael Montiel
刊名:	PLOS ONE
ISSN:	1932-6203
出版年:	2014
发表日期:	2014-8-6
卷:	9, 期:8
语种:	英语
英文关键词:	Polymerase chain reaction ; Melting ; Sex determination ; Osteology ; Ancient DNA ; DNA extraction ; Y chromosomes ; Gene amplification
英文摘要:	Sex identification in ancient human remains is a common problem especially if the skeletons are sub-adult, incomplete or damaged. In this paper we propose a new method to identify sex, based on real-time PCR amplification of small fragments (61 and 64 bp) of the third exon within the amelogenin gene covering a 3-bp deletion on the AMELX-allele, followed by a High Resolution Melting analysis (HRM). HRM is based on the melting curves of amplified fragments. The amelogenin gene is located on both chromosomes X and Y, showing dimorphism in length. This molecular tool is rapid, sensitive and reduces the risk of contamination from exogenous genetic material when used for ancient DNA studies. The accuracy of the new method described here has been corroborated by using control samples of known sex and by contrasting our results with those obtained with other methods. Our method has proven to be useful even in heavily degraded samples, where other previously published methods failed. Stochastic problems such as the random allele drop-out phenomenon are expected to occur in a less severe form, due to the smaller fragment size to be amplified. Thus, their negative effect could be easier to overcome by a proper experimental design.
URL:	http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0104629&type=printable
Citation statistics:
资源类型:	期刊论文
标识符:	http://119.78.100.158/handle/2HF3EXSE/18625
Appears in Collections:	过去全球变化的重建影响、适应和脆弱性科学计划与规划气候变化与战略全球变化的国际研究计划气候减缓与适应气候变化事实与影响

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File Name/ File Size	Content Type	Version	Access	License
journal.pone.0104629.PDF（575KB）	期刊论文	作者接受稿	开放获取	CC BY-NC-SA	View Download

作者单位:

Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico;Instituto de Investigaciones Antropológicas, Universidad Nacional Autónoma de México, Mexico City, Mexico;Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Guanajuato, Mexico

Recommended Citation:

Brenda A. Álvarez-Sandoval,Linda R. Manzanilla,Rafael Montiel. Sex Determination in Highly Fragmented Human DNA by High-Resolution Melting (HRM) Analysis[J]. PLOS ONE,2014-01-01,9(8)

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