In order to reduce the ecotoxicity of paper mill, four different enzymatic pretreatment strategies were investigated in comparison to conventional chemical based processes. In strategy I, xylanase-aided pretreatment of pulp was carried out, and in strategy II, xylanase and laccase-mediator systems were used sequentially. Moreover, to compare the efficiency of Bacillus stearothermophilus xylanase and Ceriporiopsis subvermispora laccase in the reduction of ecotoxicity and pollution, parallel strategies (III and IV) were implemented using commercial enzymes. Conventional CDEOPD1D2 (CD, Cl2 with ClO2; EOP, H2O2 extraction; D1 and D2, ClO2) and X/XLCDEOPD1D2 (X, xylanase; L, laccase) sequences were employed with non-enzymatic and enzymatic strategies, respectively. Acute toxicity was determined by the extent of inhibition of bioluminescence of Vibrio fischeri with different dilutions of the effluent. Two-fold increase was observed in EC50 values for strategy I compared to the control process. On the other hand, sequential application of commercial enzymes resulted in higher acute toxicity compared to lab enzymes. In comparison to the control process, strategy II was the most efficient and successfully reduced 60.1 and 25.8% of biological oxygen demand (BOD) and color of effluents, respectively. We report for the first time the comparative analysis of the ecotoxicity of industrial effluents.
Department of Chemical Engineering, Konkuk University, Gwangjin–Gu, Seoul, Republic of Korea;Institute of SK–KU Biomaterials, Konkuk University, Gwangjin–Gu, Seoul, Republic of Korea;Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India;Department of Biotechnology, Maharishi Markandeshwar University, Mullana-Ambala, Haryana, India;Department of Biotechnology, Kurukshetra University, Kurukshetra, Haryana, India;Microbial Biotechnology and Genomics, CSIR-Institute of Genomics and Integrative Biology, Delhi University Campus, Delhi, India;Department of Chemical Engineering, Konkuk University, Gwangjin–Gu, Seoul, Republic of Korea;Department of Chemical Engineering, Konkuk University, Gwangjin–Gu, Seoul, Republic of Korea;Institute of SK–KU Biomaterials, Konkuk University, Gwangjin–Gu, Seoul, Republic of Korea
Recommended Citation:
Saurabh Sudha Dhiman,Gaurav Garg,Jitender Sharma,et al. Reduction in Acute Ecotoxicity of Paper Mill Effluent by Sequential Application of Xylanase and Laccase[J]. PLOS ONE,2014-01-01,9(7)